Search for dissertations about: "light-up probes"

Found 3 swedish dissertations containing the words light-up probes.

2. 1. The Light-up Probe

   Author : Nicke Svanvik; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

   Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE

4. 2. Further development of light-up probes : dye synthesis and PCR applications

   Author : Jennie Isacsson; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; homogeneous assays; flourecent hybridisation probes; light-up probes; peptide nucleic acids; PCS; solid phase synthesis; LightUp probe;

   Abstract : .... READ MORE

5. 3. Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions

   Author : Anke Dierckx; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; FRET; B-DNA; circular dichroism; fluorescent nucleobase analogue; adenine analogue; duplex stability; Fluorescence quantum yield;

   Abstract : Ever since unravelling the structure of DNA, an expanding research field has emerged with ongoing efforts dedicated to increase our understanding of the molecule of life. Since the natural nucleobases are virtually non-emissive, it has been a challenge for decades to ‘light up’ DNA/RNA in order to investigate their properties utilizing fluorescence techniques. READ MORE