Search for dissertations about: "lipase production and purification"

Found 5 swedish dissertations containing the words lipase production and purification.

  2. 1. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Author : Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Abstract : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. READ MORE

  4. 2. Enterostatin in the Gastrointestinal Tract - Production and Possible Mechanism of Action

    Author : Maria Sörhede Winzell; Lund Medicin; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Physiology; insulin secretion; neuropeptide; lipolysis; appetite; Colipase; fat intake; Fysiologi;

    Abstract : The presence of procolipase and enterostatin in the rat gastrointestinal tract has been investigated. Procolipase was identified, using in situ hybridization and immunohistochemistry, in exocrine cells of the pancreas as well as in chief cells of the fundus region of the stomach. READ MORE

  5. 3. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Author : Malin Eklund; KTH; []
    Keywords : phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Abstract : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. READ MORE

  6. 4. Oxidation of Ketones: A (Chemo-) Enzymatic Approach Using Oxygenases and Hydrolases

    Author : Georgina Chávez; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; cross linked enzyme aggregates.; caprolactone; perhydrolase; Lipase B from Candida antarctica;

    Abstract : Oxidation reactions are important in organic chemistry as well as in nature. In industry, oxidations are commonly used for the synthesis of chemicals and pharmaceuticals, however such processes have a number of limitations, they use chlorinated solvents, stoichiometric oxidation reagents, and in some cases the reagents that have risks of explosion during transportation and storage. READ MORE

  7. 5. Expression and Mutagenesis studies of Candida antactica lipase B

    Author : Johanna C. Rotticci-Mulder; KTH; []
    Keywords : Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Abstract : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. READ MORE