Search for dissertations about: "low affinity interactions"

Showing result 6 - 10 of 85 swedish dissertations containing the words low affinity interactions.

  2. 6. On the application and validation of multiplexed affinity assays

    Author : Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Bioteknologi; Biotechnology;

    Abstract : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. READ MORE

  4. 7. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

    Author : Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

    Abstract : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. READ MORE

  5. 8. Affinity based proteomics research tools

    Author : Cecilia Eriksson; Sophia Hober; Michael Taussig; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Immunology; Immunologi; Molecular biology; Molekylärbiologi;

    Abstract : Listen to the mantra; the mapping of the genome was finished in 2001, and the sequel research challenge is the thorough survey of the corresponding human proteome. This was stated almost a decade ago, it has been repeated over and over, and is still most certainly a hard nut to crack. READ MORE

  6. 9. Cellular Dyes and Bioactive Peptides - Cell Membrane Interactions and Cellular Uptake

    Author : Maria Matson Dzebo; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Membrane Interactions; Spectroscopy; Cell-Penetrating Peptides; Bioactive Peptides; Cell Membrane; Confocal Laser Scanning Microscopy; Cellular Uptake; AF-16; Voltage-Sensitive Dyes; Cell Dyes; Antisecretory Factor; Ruthenium Complexes; Isothermal Titration Calorimetry; Flow-Cytometry;

    Abstract : Knowledge about mechanisms behind interactions of molecules with biomembranes and cellular uptake is very important for understanding biological processes and for drug design. The work described in this thesis has focused on interactions of cellular dyes and bioactive peptides with cells and the cell membrane. READ MORE

  7. 10. Revealing Secrets of Synaptic Protein Interactions : A Biosensor based Strategy

    Author : Christian Seeger; U Helena Danielson; Sarah Lummis; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Surface plasmon resonance; biosensor; AMPA receptor; GABAA receptor; ligand-gated ion channel; A-kinase anchoring protein; caldendrin; calmodulin; HIV; fragment based drug discovery; Biokemi; Biochemistry; Neuroscience; Neurovetenskap;

    Abstract : Protein interactions are the basis of synaptic function, and studying these interactions on a molecular level is crucial for understanding basic brain function, as well as mechanisms underlying neurological disorders. In this thesis, kinetic and mechanistic characterization of synaptic protein interactions was performed by using surface plasmon resonance biosensor technology. READ MORE