Search for dissertations about: "low copy nuclear DNA"

Showing result 1 - 5 of 10 swedish dissertations containing the words low copy nuclear DNA.

2. 1. Disentangling the Reticulate History of Polyploids in Silene (Caryophyllaceae)

   Author : Magnus Popp; Bengt Oxelman; Magnus Lidén; Jeff J. Doyle; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; Silene; Sileneae; polyploidy; low copy nuclear DNA; RNA polymerase; RPA2; RPB2; RPD2; internal transcribed spacer; rps16; psbE-petL; concerted evolution; reticulate evolution; lineage sorting; Biologi; Biology; Biologi;

   Abstract : DNA sequences from the rps16 intron and the psbE-petL spacer from the chloroplast genome, the ribosomal nuclear ITS region, and introns from the low copy nuclear genes RPA2, RPB2, RPD2a and RPD2b, are in different combinations used to infer phylogenetic relationships in Sileneae (Caryophyllaceae). Used in concert, the biparentally inherited nuclear regions are useful to distinguish between paralogy due to allopolyploidy and single gene duplications, respectively, because the latter are not expected to give rise to repeated phylogenetic patterns in potentially unlinked sequence regions. READ MORE

4. 2. Molecular mechanism of plasmid copy number control in Yersinia

   Author : Tifaine Héchard; Helen Wang; Javier Pizarro-Cerda; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia pseudotuberculosis; plasmid copy number; T3SS; infection; ddPCR; Mikrobiologi; Microbiology;

   Abstract : The ability of pathogenic bacteria to cause disease depends on various virulence mechanisms. The three pathogenic species of Yersinia use a type III secretion system (T3SS) to translocate effector proteins into host cells and disrupt the immune system. This T3SS is encoded on a 70kb, low-copy, virulence plasmid. READ MORE

5. 3. Application of proximity Ligation for Detection of Proteins, Biomolecular Interactions, and Single Copies of Pathogens

   Author : Sigrun Margret Gustafsdottir; Ulf Landegren; Per-Åke Nygren; Uppsala universitet; []
   Keywords : Molecular medicine; Proximity ligation; Cytokines; Microbial pathogens; Protein-DNA interactions; Drug screening; Molekylärmedicin;

   Abstract : Proximity ligation is a recently established technique that can provide answers to questions about the concentration, localization, interactions, modifications and functions of proteins. The method enables sensitive protein measurements with a detection limit in the low femtomolar range in complex biological samples. READ MORE

6. 4. Phylogenies and Secondary Chemistry in Arnica (Asteraceae)

   Author : Catarina Ekenäs; Katarina Andreasen; Anders Backlund; Lynn Bohs; Uppsala universitet; []
   Keywords : Biology; phylogeny; ITS; ETS; RPB2; sesquiterpene lactones; PCA; bioassay; NF-κB; neutrophil; chloroplast; Biologi; Arnica; Asteraceae;

   Abstract : The genus Arnica (Asteraceae) was investigated for phylogenetic relationships and sesquiterpene lactone (STL) content with the aims to trace the evolutionary history of the genus and to investigate possible congruencies between DNA sequence data, secondary chemistry, and biological activity. Complex evolutionary patterns in Arnica are evident from phylogenetic analyses of chloroplast regions (the rpl16 and rps16 introns and the psbA–trnH, ycf4–cemA, and trnT–L spacers), nuclear ribosomal regions (the internal and external transcribed spacers) and the nuclear low-copy DNA region coding for the second largest subunit of RNA polymerase II (RPB2) between exons 17 and 23. READ MORE

7. 5. Bridging the past to the present: investigating species boundaries with herbarium specimens and next-generation-sequencing. A case study of circumpolar Silene sect. Physolychnis

   Author : Anne-Sophie Quatela; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; allele phasing; Bayesian inference; herbarium specimen; long read sequencing; multispecies coalescent; Silene; systematics; target capture; species complexes;

   Abstract : During the recent decades, genetic information enclosed in herbarium collections have been partly revealed, largely due to the fast-moving high-throughput sequencing technologies. However, the genetic outcome from herbarium material is limited by its degradation over time, often worsened by certain preservation methods. READ MORE