Search for dissertations about: "luciferase"

Showing result 1 - 5 of 92 swedish dissertations containing the word luciferase.

2. 1. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

   Author : Jonas Eriksson; KTH; []
   Keywords : bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

   Abstract : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. READ MORE

4. 2. Recombinant Enzymes in Pyrosequencing Technology

   Author : Nader Nourizad; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

5. 3. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors

   Author : Knut Kotarsky; Slemhinnans immunologi; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemi; Metabolism; Biochemistry; metabolism; leukotrieneB4; thiazolidinedione; 9-HODE; fatty acid; luciferase; GFP; reporter gene; preclinical assay; receptor; GPCR;

   Abstract : Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of ligands. They also represent important drug targets. READ MORE

6. 4. Evaluating an experimental model consecutive to abdominal wall hernia repair outcome

   Author : Annika Winsnes; Karin Strigård; Ulf Gunnarsson; Peter Falk; Birgit Stark; Henrik Thorlacius; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hernia; mesh; luciferase; full thickness skin grafts; mice; IPOM; onlay; reinforcement materials; abdominal wall; Bråck; nät; luciferas; fullhudstransplantat; FTSG; möss; IPOM; onlay; förstärkningsmaterial; bukvägg; Surgery; kirurgi;

   Abstract : Background: Abdominal wall hernia is a common surgically treated condition. Patients with primary umbilical hernia are operated with suture or mesh repair, but recurrence and complication rates have been debated.Larger abdominal wall hernias need implantation of reinforcing material for repair. Synthetic implants are dominating. READ MORE

7. 5. Molecular Pharmacology and Structure Function Modelling of the Leukotriene B4 Receptor BLT1

   Author : Alan Sabirsh; Institutionen för experimentell medicinsk vetenskap; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; pharmacognosy; pharmacy; toxicology; Farmakologi; farmakognosi; farmaci; toxikologi; Pharmacological sciences; fluidity; redox; chemotaxis; luciferase; calcium; ligand binding; point mutation; modelling; antibody; BLT1; leukotriene; pharmacology; GPCR; G-protein; receptor;

   Abstract : Leukotrienes are membrane derived bioactive lipids that play an important role in immune responses by initiating and maintaining the inflammatory responses. Leukotriene B4 (LTB4) released at the site of an inflammatory response attracts, activates and prolongs the life of leukocytes and lymphocytes. READ MORE