Search for dissertations about: "mRNA secondary structure"

Showing result 6 - 10 of 20 swedish dissertations containing the words mRNA secondary structure.

  2. 6. Lead(II) as a Tool for Probing RNA Structure in vivo

    Author : Magnus Lindell; Gerhart Wagner; Roland Hartmann; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; lead II cleavage; RNA structure; in vivo probing; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Abstract : Chemical modification and limited enzymatic hydrolysis are powerful methods to obtain detailed information on the structure and dynamics of RNAs in solution. In the work presented here I have taken advantage of the properties of the divalent metal ion lead(II) to establish it as a new probe for investigating the structure of RNA in vivo. READ MORE

  4. 7. Fungal Lectins. Molecular structure and function of a member of a novel lectin family

    Author : Stefan Rosén; Biologiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; possible functions; expression; localization; binding specificities; primary and secondary structure; Agaricus bisporus; Arthrobotrys oligospora; Fungi; novel lectin family; Biology; Biologi;

    Abstract : Lectins defined as non-enzyme, non-immunoglobulin carbohydrate binding proteins, have been found in a wide variety of viruses, bacteria, fungi, plants and animals. Although lectins have been purified from more than 60 different species of fungi, the structure and biological functions of these proteins are not well known. READ MORE

  5. 8. Expression and structure-function characterisation of herpesviral proteins

    Author : Sue-Li Dahlroth; Pär Nordlund; Darren Hart; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein production; Herpesvirus; SOX; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : In order to determine and study a protein structure, large amounts of it is needed. The easiest way to obtain a protein is to recombinantly overexpress it in the well-studied bacterium Escherichia coli. However, this expression host has one major disadvantage, overexpressed proteins might not be folded or be insoluble. READ MORE

  6. 9. Kinetic assays for RNA-cleaving deoxyribozymes and other nucleases

    Author : Jonas Eriksson; Ülo Langel; Pia Ädelroth; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Abstract : In this thesis two different assays for real-time RNA-cleaving deoxyribozyme and general nuclease kinetics are presented. Previous publications on nuclease kinetic assays have been riddled with drawbacks of labeling, discontinuity, cost etc. READ MORE

  7. 10. The Involvement of the structures of antisense RNA, target RNA, and their complexes in plasmid R1 copy number regulation

    Author : Charlotta Ingvoldstad; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

    Abstract : Plasmid R1 is a low-copy-number plasmid which belongs to the incompatibility group FII. R1 regulates its own copy number. Initiation of replication of plasmid R1 requires the binding of the RepA protein to oriR1. RepA acts in cis and has to be synthesized de novo for each round of replication. READ MORE