Search for dissertations about: "mammalian expression"

Showing result 1 - 5 of 356 swedish dissertations containing the words mammalian expression.

  2. 1. Gene silencing and expression during mammalian development : The special case of the imprinted H19 gene

    Author : Kristian Svensson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; Biologi; Biology; Biologi; zoologisk utvecklingsbiologi; Animal Development;

    Abstract : Embryonic development consists of a vast number of fine-tuned, complex processesthat organise the body plan and individual organs. To enable developmental decisions,genes pivotal for normal development must be stably activated and silenced, asappropriate. READ MORE

  4. 2. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    Author : Viveka Dolby; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Abstract : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. READ MORE

  5. 3. Sexually Dimorphic Gene Expression in the Mammalian Brain

    Author : Björn Reinius; Elena Jazin; Klas Kullander; Arthur Arnold; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; Utvecklingsbiologi;

    Abstract : In recent times, major advances have been made towards understanding sexual dimorphism in the brain on a molecular basis. This thesis summarises my modest contributions to these endeavours. Sexual dimorphisms are manifested throughout the spectrum of biological complexity, and can be studied by numerous approaches. READ MORE

  6. 4. Regulation of site-selective A-to-I RNA editing : During mammalian brain development

    Author : Helene Wahlstedt; Marie Öhman; Mary O'connell; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; RNA editering; ADAR; brain development; Molecular biology; Molekylärbiologi; Molecular Biology; molekylärbiologi;

    Abstract : Adenosine (A) to inosine (I) RNA editing is a widespread post-transcriptional mechanism in mammals that contributes to increase the protein diversity. Adenosine deaminases that act on RNA (ADARs) are the enzymes catalyzing RNA editing. ADARs are particularly active within the brain where they act on transcripts involved in neurotransmission. READ MORE

  7. 5. Production and delivery of recombinant subunit vaccines

    Author : Christin Andersson; KTH; []
    Keywords : Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Abstract : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. READ MORE