Search for dissertations about: "massive parallel sequencing"

Showing result 1 - 5 of 14 swedish dissertations containing the words massive parallel sequencing.

  2. 1. Tagging systems for sequencing large cohorts

    Author : Mårten Neiman; Afshin Ahmadian; Peter Savolainen; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; next generation sequencing; genotyping; massive parallel sequencing; 454; Pyrosequencing; amplicon sequencing; enrichment; DNA barcodes; Genetics; Genetik;

    Abstract : Advances in sequencing technologies constantly improves the throughput andaccuracy of sequencing instruments. Together with this development comes newdemands and opportunities to fully take advantage of the massive amounts of dataproduced within a sequence run. READ MORE

  4. 2. Enabling massive genomic and transcriptomic analysis

    Author : Henrik Stranneheim; Joakim Lundeberg; Elaine Mardis; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; DNA; RNA; sequencing; massively parallel sequencing; alignment; assembly; single nucleotide polymorphism; LHON;

    Abstract : In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA. This has revolutionized the fields of genetics and biology, leading to a deeper understanding of the molecular events in life processes. READ MORE

  5. 3. Barcoded DNA Sequencing for Parallel Protein Detection

    Author : Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

    Abstract : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. READ MORE

  6. 4. Massively parallel analysis of cells and nucleic acids

    Author : Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

    Abstract : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. READ MORE

  7. 5. Applying massive parallel sequencing technologies to unravel the genomic complexity in domestic species

    Author : Agnese Viluma; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : NATURVETENSKAP; LANTBRUKSVETENSKAPER; LANTBRUKSVETENSKAPER; NATURAL SCIENCES; AGRICULTURAL SCIENCES; AGRICULTURAL SCIENCES;

    Abstract : Massive parallel sequencing technologies have made a remarkable contribution in understanding the genomic complexity in human and other mammalian species. This thesis includes four studies illustrating the technology change from short-read to long-read massive parallel sequencing and the corresponding layers of genomic complexity resolved in dog and horse. READ MORE