Search for dissertations about: "massively parallel sequencing"

Showing result 1 - 5 of 30 swedish dissertations containing the words massively parallel sequencing.

2. 1. Library Preparation for High Throughput DNA Sequencing

   Author : Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Biotechnology; Bioteknologi;

   Abstract : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. READ MORE

4. 2. Genetic Cartography at Massively Parallel Scale

   Author : Johan Dahlberg; Ann-Christine Syvänen; Adam Ameur; Michael C. Zody; Uppsala universitet; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; MEDICAL AND HEALTH SCIENCES; MEDICAL AND HEALTH SCIENCES; Acute Lymphoblastic Leukemia ALL ; RNA-Sequencing; Bioinformatics; Pooling; Whole Genome Sequencing; Medicinsk genetik; Medical Genetics; Bioinformatics; Bioinformatik;

   Abstract : Massively parallel sequencing (MPS) is revolutionizing genomics. In this work we use, refine, and develop new tools for the discipline.MPS has led to the discovery of multiple novel subtypes in Acute Lymphoblastic Leukemia (ALL). In Study I we screen for fusion genes in 134 pediatric ALL patients, including patients without an assigned subtype. READ MORE

5. 3. Methods for Analyzing Genomes

   Author : Patrik L. Ståhl; Joakim Lundeberg; Ivo Gut; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; array; sequence capture; genotyping; trinucleotide threading; sequencing; massively parallel sequencing; single molecule sequencing; Visual DNA; p53; single nucleotide polymorphism; biomarker; Genetics; Genetik;

   Abstract : The human genome reference sequence has given us a two‐dimensional blueprint of our inherited code of life, but we need to employ modern‐day technology to expand our knowledge into a third dimension. Inter‐individual and intra‐individual variation has been shown to be larger than anticipated, and the mode of genetic regulation more complex. READ MORE

6. 4. Massively parallel analysis of cells and nucleic acids

   Author : Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

   Abstract : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. READ MORE

7. 5. Enabling massive genomic and transcriptomic analysis

   Author : Henrik Stranneheim; Joakim Lundeberg; Elaine Mardis; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; DNA; RNA; sequencing; massively parallel sequencing; alignment; assembly; single nucleotide polymorphism; LHON;

   Abstract : In recent years there have been tremendous advances in our ability to rapidly and cost-effectively sequence DNA. This has revolutionized the fields of genetics and biology, leading to a deeper understanding of the molecular events in life processes. READ MORE