Search for dissertations about: "matrix-assisted laser desorption ionization mass spectrometry MALDI-MS"

Showing result 1 - 5 of 22 swedish dissertations containing the words matrix-assisted laser desorption ionization mass spectrometry MALDI-MS.

  2. 1. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

    Author : Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

    Abstract : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. READ MORE

  4. 2. Electrophoretic focusing in microchannels combined with mass spectrometry : Applications on amyloid beta peptides

    Author : Saara Mikkonen; Åsa Emmer; Johan Jacksén; Tapio Kotiaho; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; amyloid beta; computer simulation; electrophoresis; electrospray ionization; isoelectric focusing; isotachophoresis; MALDI; mass spectrometry; microchannel; microchip; nano-electrospray ionization; preconcentration; separation; Kemi; Chemistry;

    Abstract : Analysis of low-abundance components in small samples remains a challenge within bioanalytical chemistry, and new techniques for sample pretreatments followed by sensitive and informative detection are required. In this thesis, procedures for preconcentration and separation of proteins and peptides in open microchannels fabricated on silicon microchips are presented. READ MORE

  5. 3. Tools for applied soft ionization mass spectrometry

    Author : Joakim Romson; Åsa Emmer; Peter Dinér; Steven Wilson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; Electrospray ionization; Matrix assisted laser desorption ionization; Mass accuracy; Mass shifts; Ion trap; Capillary electrophoresis; Sample concentration; Masspektrometri; Elektrospray-jonisering; Matrisassisterad laser desorbtion jonisering; Mass-exakthet; Masskift; Jonfälla; Kapillärelektrofores; Provkoncentration; Kemi; Chemistry;

    Abstract :  Mass spectrometry (MS) is an important analytical tool. Its most importantadvantages are sensitivity, resolution, and accurate and precise m/zmeasurements. To get the best results from an instrument, the user musthave knowledge of its limitation and requirements. READ MORE

  6. 4. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

    Author : Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

    Abstract : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. READ MORE

  7. 5. Development of Methods for Protein and Peptide Analysis Applied in Neuroscience Utilizing Mass Spectrometry

    Author : Johan Pierson; Per Andrén; Ulf Bondesson; György Marko-Varga; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Mass spectrometry; Parkinson’s disease; Metabolism; Profiling Mass Spectrometry; Proteomics; Analytisk kemi; Analytical chemistry; Analytisk kemi;

    Abstract : This thesis describes the utilization of the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) and electrospray ionization (ESI) MS techniques for analysis of complex brain tissue samples. Direct molecular profiling of biological samples using MALDI MS is a powerful tool for identifying phenotypic markers. READ MORE