Search for dissertations about: "membrane monosaccharides IPED ion spray probe internal geometry extraction fraction electrochemical"

Found 1 swedish dissertation containing the words membrane monosaccharides IPED ion spray probe internal geometry extraction fraction electrochemical.

2. 1. Microdialysis Sampling, Electrochemical Detection and Mass Spectrometry of Carbohydrates in Monitoring Bioprocesses

   Author : Nelson Torto; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; monosaccharides; membrane; IPED; ion spray; probe internal geometry; extraction fraction; electrochemical oxidation; electrochemical detection; disaccharides; desalting; carbohydrates; bioprocess monitoring; Cu; APCI; Au; MS; oligosaccharides; PAD; PEI; Analytical chemistry; Analytisk kemi;

   Abstract : This thesis addresses; (1) the microdialysis experiment, application and methodology to maximise its performance when sampling from enzymatic bioprocesses, (2) electrochemical detection and electrochemical oxidation of carbohydrates on Au as well as Cu electrodes in alkaline solutions, and (3) coupling and maximising the performance of a microdialysis sampling based high performance anion exchange chromatography-integrated pulsed electrochemical detection-mass spectrometry system. Studies were carried out to investigate the effect of temperature, analyte as well as biomatrix complexity, analyte concentration, permeability factors, protein(enzyme)-membrane interaction and membrane material on the performance of microdialysis sampling. READ MORE