Search for dissertations about: "metabolic flux analysis"

Showing result 1 - 5 of 38 swedish dissertations containing the words metabolic flux analysis.

  1. 1. Metabolic analysis of shikimic acid producing Escherichia coli

    Author : Louise Johansson; Institutionen för kemiteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

    Abstract : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. READ MORE

  2. 2. On metabolic networks and multi-omics integration

    Author : Rasmus Ågren; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; succinate; genome-scale metabolic model; metabolic engineering; systems biology; penicillin; NASH; flux balance analysis; metabolism; cancer; reconstruction;

    Abstract : Cellular metabolism is a highly complex chemical system, involving thousands of interacting metabolites and reactions. The traditional approach to understanding metabolism has been that of reductionism; by isolating and carefully measuring the involved components, the goal has been to understand the whole as the sum of its parts. READ MORE

  3. 3. Macroscopic models of Chinese hamster ovary cell cultures

    Author : Erika Hagrot; Véronique Chotteau; Anders Forsgren; Nathan E. Lewis; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; Chinese hamster ovary; Amino acid; Metabolic network; Metabolic flux analysis; Kinetic modeling; Elementary flux mode; Optimization; Column generation; Bioteknologi; Biotechnology;

    Abstract : Biopharmaceuticals treat a range of diseases, and is a growing sector within the pharmaceutical industry. A majority of these complex molecules are produced by genetically modified mammalian cells in large-scale cell cultures. READ MORE

  4. 4. Macroscopic Modeling of Metabolic Reaction Networks and Dynamic Identification of Elementary Flux Modes by Column Generation

    Author : Hildur Æsa Oddsdóttir; Anders Forsgren; Bernhard Palsson; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Metabolic Network; Optimization; Robust Optimization; Least-squares; Column Generation; Modeling; Algorithm; Elementary Flux Mode; Metabolic Flux Analysis; Chinese Hamster Ovary Cell; Amino Acid Metabolism; Tillämpad matematik och beräkningsmatematik; Applied and Computational Mathematics;

    Abstract : In this work an intersection between optimization methods and animal cell culture modeling is considered. We present optimization based methods for analyzing and building models of cell culture; models that could be used when designing the environment cells are cultivated in, i.e., medium. READ MORE

  5. 5. Analysis and Control of Continuous Microaerobic Ethanol Production by Yeast

    Author : Carl Johan Franzén; Chalmers University of Technology; []
    Keywords : nitrogen limitation; ethanol; Saccharomyces cerevisiae; glycerol; metabolic flux analysis; oxygen limitation; stoichiometric model; yeast physiology; RQ control;

    Abstract : Glucose fermentation by Saccharomyces cerevisiae, and xylose fermentation by Candida utilis and Pichia stipitis, were investigated under microaerobic conditions. A dynamic experimental method named oxygen programmed fermentation (OPF), and a method for microaerobic RQ control in continuous culture have been developed. READ MORE