Search for dissertations about: "metabolite screening"

Showing result 1 - 5 of 25 swedish dissertations containing the words metabolite screening.

2. 1. Toxicometabolomics and biotransformation product screening in single zebrafish embryos

   Author : Anton Ribbenstedt; Jonathan P. Benskin; Magnus Breitholtz; Elena Gorokhova; Emma Schymanski; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Non-target; REACH; Orbitrap; single embryo; zebrafish embryo; metabolism; high throughput; hydroxylation; glucuronidation; metabolite screening; phase I; phase II; pharmaceutical; structure elucidation; conjugation; propranolol; beta blocker; Non-targeted; toxicometabolomics; Orbitrap; single embryo; 3R; metabolomics; MoA; mode of action; zebrafish embryo; carbamazepine; biotransformation; Applied Environmental Science; tillämpad miljövetenskap;

   Abstract : Over the last decade environmental agencies worldwide have escalated their work to phase out animal testing for the purposes of chemical regulation. Meanwhile the number of commercially available chemicals and the requirements for hazard assessments have both increased, creating a large need for substitution of traditional in vivo assays with in vitro tests. READ MORE

4. 2. Metabolite biosensors for cell factory development

   Author : Yasaman Dabirian; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; malonyl-CoA; metabolite-responsive transcription factor-based biosensors; Saccharomyces cerevisiae; alkanes; fatty acyl-CoA; synthetic biology;

   Abstract : Through synergy with natural sciences and engineering disciplines, biotechnology has become a broad, interdisciplinary, scientific field with many applications. One such application is the sustainable production of industrially relevant products using living systems such as microorganisms. READ MORE

5. 3. Multi-platform metabolomics assays to study the responsiveness of the human plasma and lung lavage metabolome

   Author : Masoumeh Karimpour; Malin Nording L.; Magnus Åberg; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; LC MS; GC MS; NMR; metabolomics; metabolite; exposure; postprandial; plasma; lung lavage; BW; BW; data analysis; univariate analysis; multivariate analysis;

   Abstract : Metabolomics as a field has been used to track changes and perturbations in the human body by investigating metabolite profiles indicating the change of metabolite levels over time and in response to different challenges. In this thesis work, the main focus was on applying multiplatform-metabolomics to study the human metabolome following exposure to perturbations, such as diet (in the form of a challenge meal) and exhaust emissions (air pollution exposure in a controlled setting). READ MORE

6. 4. CRISPR based technologies for high-throughput metabolic engineering of yeast

   Author : Christos Skrekas; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

   Abstract : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. READ MORE

7. 5. Multivariate profiling of metabolites in human disease : Method evaluation and application to prostate cancer

   Author : Elin Thysell; Henrik Antti; Janne Lehtiö; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; metabolite profiling; metabolomics; predictive metabolomics; mass spectrometry; GC-MS; biomarkers; chemometrics; design of experiments; multivariate data analysis; prostate cancer; bone metastases; plasma; biologisk kemi; biological chemistry;

   Abstract : There is an ever increasing need of new technologies for identification of molecular markers for early diagnosis of fatal diseases to allow efficient treatment. In addition, there is great value in finding patterns of metabolites, proteins or genes altered in relation to specific disease conditions to gain a deeper understanding of the underlying mechanisms of disease development. READ MORE