Search for dissertations about: "modified nucleotides"

Showing result 1 - 5 of 25 swedish dissertations containing the words modified nucleotides.

2. 1. Oxidized nucleotides as a predictor of radiation sensitivity

   Author : Ali Pour Khavari; Siamak Haghdoost; Marie Öhman; Anders Nilsson; Bo Stenerlöw; Neus Visa; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; 8-oxo-dG; Radiation; Sensitivity; Oxidized; Nucleotides; ROS; NOS; molekylär biovetenskap; Molecular Bioscience;

   Abstract : The direct and indirect effects of IR can lead to DNA damage and activation of DNA repair. The indirect effects of ionizing radiation mediated by reactive oxygen species (ROS) which produced through radiolysis of water and if not taken care of by the antioxidant system, can also give rise to oxidative stress. READ MORE

4. 2. Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides : Design, Synthesis and Properties

   Author : Dmytro Honcharenko; Jyoti Chattopadhyaya; Piet Herdewijn; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; antisense oligonucleotides; conformationally constrained nucleosides; azetidine; aza-ENA; target affinity; RNase H; exonuclease stability; pyrene-functionalized nucleotides; fluorescence; mismatch discrimination; Bioorganisk kemi; Cell and molecular biology; Cell- och molekylärbiologi;

   Abstract : This thesis is based on six original research publications describing synthesis, structure and physicochemical and biochemical analysis of chemically modified oligonucleotides (ONs) in terms of their potential diagnostic and therapeutic applications. Synthesis of two types of bicyclic conformationally constrained nucleosides, North-East locked 1',2'-azetidine and North locked 2',4'-aza-ENA, is described. READ MORE

5. 3. Studies on Nucleic Acids – Structure and Dynamics

   Author : Johan Isaksson; Jyoti Chattopadhyaya; Douglas H. Turner; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; nucleic acids; modified nucleotides; NMR; structure; dangling-end; single-stranded; thermodynamics; hydration; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi;

   Abstract : This thesis is based on six papers, Papers I-VI, focusing on the interplay between the stabilizing elements of nucleic acids self-assembly; hydrogen bonding, stacking and solvent effects. In Paper I we investigate how the substitution of the O4' for CH2 in the sugar moiety of adenosine (2'-deoxyaristeromycin) at the A6 position of the Dickerson-Drew dodecamer makes the two modified bases exist in a dynamic equilibrium between Hoogsteen and Watson-Crick base pairing in the NMR time scale. READ MORE

6. 4. Development of Electrical Readouts for Amplified Single Molecule Detection

   Author : Camilla Russell; Mats Nilsson; Björn Högberg; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; metallization; electrical DNA detection; magnetic nanoparticles; dielectrophoresis; boranephosphonate modified nucleotides; DNA elongation; Biokemi; Biochemistry;

   Abstract : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. READ MORE

7. 5. Wobble modifications and other features in transfer RNA important for decoding and reading frame maintenance

   Author : Joakim Näsvall; Glenn Björk; Michael O'Connor; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transfer RNA; Modified nucleosides; Decoding; Reading frame maintenance; Molecular biology; Molekylärbiologi;

   Abstract : Transfer RNA (tRNA) is the adaptor molecule responsible for bringing the correct amino acid to the ribosome during protein synthesis. tRNA contains a number of modified nucleosides, which are derivatives of the four normal nucleosides. READ MORE