Search for dissertations about: "molten globule"

Showing result 1 - 5 of 23 swedish dissertations containing the words molten globule.

  2. 1. Molecular principles of protein stability and protein-protein interactions

    Author : Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

    Abstract : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. READ MORE

  4. 2. Structural Transitions in Helical Peptides : The Influence of Water – Implications for Molecular Recognition and Protein Folding

    Author : Martin Lignell; Hans-Christian Becker; Kaare Teilum; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein dynamics; protein folding; molten globule; time-resolved fluorescence spectroscopy; CD spectroscopy; molecular recognition; structure-function paradigm; Chemical physics; Kemisk fysik; Physical Chemistry; Fysikalisk kemi;

    Abstract : Fluctuations in protein structure are vital to function. This contrasts the dominating structure-function paradigm, which connects the well-defined three-dimensional protein structure to its function. However, catalysis is observed in disordered enzymes, which lack a defined structure. READ MORE

  5. 3. Conformational changes in Staphylococcus aureus' alpha-toxin : from water-soluble monomer to a transmembrane channel

    Author : Beatrix Vécsey Semjén; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Staphylococcal alpha-toxin membrane-protein interaction pore formingtoxins.;

    Abstract : Alpha-toxin is secreted by Staphylococcus aureus as a soluble monomer that without further activation can oligomerize and form a transmembrane channel. One of the aims of the present work was to understand the underlying mechanisms of the membrane insertion process. READ MORE

  6. 4. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

    Author : Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

    Abstract : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. READ MORE

  7. 5. Protein Misfolding in Human Diseases

    Author : Karin Almstedt; Per Hammarström; Daniel Otzen; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Misfolding; carbonic anhydrase; prion protein; protein stability; Biochemistry; Biokemi;

    Abstract : There are several diseases well known that are due to aberrant protein folding. These types of diseases can be divided into three main categories:Loss-of-function diseasesGain-of-toxic-function diseasesInfectious misfolding diseases Most loss-of-function diseases are caused by aberrant folding of important proteins. READ MORE