Search for dissertations about: "mutation rate"

Showing result 16 - 20 of 178 swedish dissertations containing the words mutation rate.

2. 16. Dynamics and Mechanisms of Adaptive Evolution in Bacteria

   Author : Song Sun; Dan Andersson; Diarmaid Hughes; Josep Casadesús; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; adaptive evolution; mutation; genome rearrangements; antibiotic resistance; gene amplification; genome reduction; directed evolution; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

   Abstract : Determining the properties of mutations is fundamental to understanding the mechanisms of adaptive evolution. The major goal of this thesis is to investigate the mechanisms of bacterial adaptation to new environments using experimental evolution. READ MORE

4. 17. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Author : Zihe Liu; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Abstract : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. READ MORE

5. 18. Arrayed identification of DNA signatures

   Author : Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

   Abstract : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. READ MORE

6. 19. Geographic and genetic diversity of hepatitis B

   Author : Erik Alestig; Göteborgs universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; hepatitis B virus; genotype; phylogeny; molecular epidemiology; sequence analysis; mutation;

   Abstract : Hepatitis B Virus (HBV) infection is a global health problem and may lead to chronic hepatitis, liver cirrhosis and hepatocellular cancer. The ability of HBV to adapt to the host environment by genetic variation has lead to the evolution of 8 established (A-H) and 2 putative genotypes (I-J), each corresponding to a rather well-defined geographical distribution. READ MORE

7. 20. Rates and Patterns of Mutation in Microsatellite DNA

   Author : Jesper Brohede; Christian Schlötterer; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; evolutionär genetik; evolutionär genetik;

   Abstract : Sequence comparisons of orthologous microsatellite loci in cattle and sheep revealed that the substitution rate in microsatellite flanking sequences does not differ from the rate in presumably neutrally evolving intron sequences. This suggests that microsatellites are generally located in regions that are not subjected to selection. READ MORE