Search for dissertations about: "non-viral gene delivery"

Showing result 1 - 5 of 22 swedish dissertations containing the words non-viral gene delivery.

2. 1. Chitosan Polyplexes as Non-Viral Gene Delivery Systems : Structure-Property Relationships and In Vivo Efficiency

   Author : Magnus Köping-Höggård; Per Artursson; Jean-Paul Behr; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutics; gene therapy; gene delivery; non-viral; polyplex; chitosan; chitosan oligomers; polyethylenimine; plasmid DNA; lung; Galenisk farmaci; Pharmaceutics; Galenisk farmaci;

   Abstract : The subject of this thesis was to develop and optimize delivery systems for plasmid DNA (pDNA) based on biocompatible polymers, in particular chitosan, suitable for non-viral gene therapy. At the onset of this thesis, studies had reported conflicting results on the efficiency of chitosan-based gene delivery systems. READ MORE

4. 2. Characterization of nucleic acid delivery with fatty acid modified cell-penetrating peptide nanoparticle formulations

   Author : Tõnis Lehto; Ülo Langel; Patrick Midoux; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transfection; gene delivery; oligonucleotide; complexes; non-viral; in vivo; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

   Abstract : Recent advances with techniques used for manipulating gene expression have brought us to an era where various gene therapeutic approaches are becoming common therapeutic tools for many previously incurable diseases. The main factor impeding the wider translation of gene therapy is that the active pharmaceutical ingredients used for interfering with gene expression are based on nucleic acids and synthetic oligonucleotides and such molecules do not readily reach their intracellular targets due to their physicochemical properties and therefore they require delivery vectors to cross the cell membrane. READ MORE

5. 3. Dendrimer-mediated gene delivery: Effect of polymer structure on cellular response

   Author : Kristina Fant; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; gene therapy; DNA condensation; cationic polymers; toxicity; non-viral vector; drug delivery; polyplex; PAMAM dendrimers;

   Abstract : The delivery of nucleic acids to cells, in vitro and in vivo, is becoming a realistic approach for intervention with gene expression at a molecular level. It can be used to repair malfunctioning genes or to introduce new functionalities, with the most important application being clinical treatment of human diseases. READ MORE

6. 4. Enhancing the efficiency of viral and non-viral gene delivery vectors

   Author : Safa Bazaz; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Gene therapy, which involves the introduction, alteration, or deletion of genetic material within cells to treat or prevent disease, holds great promise for addressing various genetic disorders and other medical conditions. However, several challenges remain in the field, particularly concerning nucleic acid delivery into target cells. READ MORE

7. 5. Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function

   Author : Anders Wittrup; Bröstcancer-genetik; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; LL-37; heparan sulfate; glypican; endocytosis; drug delivery; DNA uptake; Cancer; cell penetrating peptides; non-viral gene delivery; organelle purification; proteoglycan; syndecan.;

   Abstract : Novel therapeutics to combat cancer are urgently needed. Most current pharmacological therapies have severe side effects and are seldom curative. Macromolecular drugs, and in particular nucleic acid based drugs, offer a potential remedy for this situation. READ MORE