Search for dissertations about: "nuclease stability"

Showing result 1 - 5 of 7 swedish dissertations containing the words nuclease stability.

  2. 1. Conformationally Constrained Oligonucleotides for RNA Targeting

    Author : Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

    Abstract : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. READ MORE

  4. 2. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

    Author : Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

    Abstract : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). READ MORE

  5. 3. Development and synthesis of oligonucleotide building blocks and conjugates for therapeutic applications

    Author : Kristina Karale; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The research and development of oligonucleotide therapeutics has been a topic of great importance in life sciences. The potential to treat rare genetic diseases can be demonstrated simply by highlighting the increasing amount of approved oligonucleotide-based drugs. READ MORE

  6. 4. Biosensing platforms using graphene based bioreactive nanostructures with various dimensions

    Author : Yuanyuan Han; Klaus Leifer; Jöns Hilborn; Hu Li; Boukherroub Rabah; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Graphene; nanoparticles; nanostructure; biosensors;

    Abstract : Nanomaterials have brought new aspects and improvements to the biosensing field due to their unique physical and chemical properties that are not shown in the bulk state. This thesis focuses on concepting, developing and testing of biosensors where nanomaterials including graphene gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) constitute the biosensors. READ MORE

  7. 5. Epigenetic Control of Mammalian Development : Studies on an imprinting control region

    Author : Liang Liu; Pärt Peterson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; Epigenetic; Genomic imprinting; DNA methylation; Igf2; H19; Insulator; CTCF; Imprinting control region; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; zoologisk utvecklingsbiologi; Animal Development;

    Abstract : A subset of autosomal genes is preferentially or exclusively expressed from one of the parental alleles. This phenomenon, termed genomic imprinting, is highlighted by the neighboring Igf2 and H19 genes, which are monoallelically expressed on opposite parental chromosomes. These features are governed by a 2. READ MORE