Search for dissertations about: "nucleic acid quantification"

Showing result 16 - 20 of 21 swedish dissertations containing the words nucleic acid quantification.

2. 16. Gene expression profiling with real-time PCR

   Author : Anders Ståhlberg; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

   Abstract : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. READ MORE

4. 17. The Light-up Probe

   Author : Nicke Svanvik; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

   Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE

5. 18. PCR detection of Streptococcus pneumoniae and Haemophilus influenzae in pneumonia patients

   Author : Guma M. K. Abdeldaim; Björn Herrmann; Jonas Blomberg; Kristoffer Strålin; Birgitta Henriques; Uppsala universitet; []
   Keywords : Lower respiratory tract infections; Pneumonia; Streptococcus pneumoniae; Haemophilus influenzae; real-time PCR;

   Abstract : PCR is a rapid, reproducible method for nucleic acid detection. However, this technology displays significant deficiencies when applied in clinical microbiology. This work’s aim was to improve current diagnostics and provide sensitive and quantitative real-time PCRs. READ MORE

6. 19. Image Analysis and Deep Learning for Applications in Microscopy

   Author : Omer Ishaq; Carolina Wählby; Bernd Rieger; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Machine learning; Deep learning; Image analysis; Quantitative microscopy; Bioimaging; Computerized Image Processing; Datoriserad bildbehandling;

   Abstract : Quantitative microscopy deals with the extraction of quantitative measurements from samples observed under a microscope. Recent developments in microscopy systems, sample preparation and handling techniques have enabled high throughput biological experiments resulting in large amounts of image data, at biological scales ranging from subcellular structures such as fluorescently tagged nucleic acid sequences to whole organisms such as zebrafish embryos. READ MORE

7. 20. The effect of chronic alcoholism on epigenetic patterns regulating gene expression and neurodegeneration in the human brain

   Author : Sofia Johansson; Karolinska Institutet; Karolinska Institutet; []
   Keywords : Alcoholism; cognition; drug dependence; brain; frontal cortex; motor cortex; LUMA; epigenetics; methylation; gene expression; endogenous controls; real-time PCR.;

   Abstract : Chronic alcoholism causes about 3.2% of all deaths worldwide. It has an association to about 60 different diseases and injuries with enormous social and economical impact. Despite these facts, very few pharmacological treatments exist and we can still not explain the mechanisms behind addiction, craving and cognitive impairments seen in the abuser. READ MORE