Search for dissertations about: "nucleic acid sequence"

Showing result 11 - 15 of 58 swedish dissertations containing the words nucleic acid sequence.

  2. 11. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Author : Simon Fredriksson; Larry Gold; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. READ MORE

  4. 12. Peptide nucleic acid conjugates as artificial ribonucleases : Cu2+ and Zn2+-dependent PNAzymes

    Author : Olivia Luige; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Synthetic modified oligonucleotides are gaining momentum as powerful therapeutic tools used in the treatment of life-threatening diseases. Degradation of disease-related RNA sequences is one of the goals achieved with oligonucleotide therapeutics, albeit the currently available technologies rely on the recruitment of endogenous enzymes, RNase H or RISC, limiting the chemical architecture of oligonucleotide drugs. READ MORE

  5. 13. Expanding the alphabet of fluorescent nucleic acid base analogues: Characterization of new members

    Author : Anke Dierckx; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescent adenine analogue; fluorescent DNA base analogue; duplex stability; Fluorescence quantum yield; circular dichroism; B-DNA;

    Abstract : Fluorescence has proven to be a powerful tool for studying structure, dynamics and interactions of biomolecules such as proteins, RNA and DNA. However, since the natural DNA/RNA nucleobases are virtually non-fluorescent, there is a need for developing fluorescent nucleic acid markers. READ MORE

  6. 14. Cell-penetrating peptides and bioactive cargoes : Strategies and mechanisms

    Author : Kalle Kilk; Ülo Langel; Michael J. Gait; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptide; delivery; Peptide nucleic acid; Biochemistry; Biokemi;

    Abstract : The cell membrane is an impermeable barrier for most macromolecules. Recently discovered cell-penetrating peptides (CPPs) have gained lot of attention because they can cross the membrane, and even more, carry cargoes with them. How CPPs enter cells is still not clear, while the delivery of different cargoes has been convincingly shown. READ MORE

  7. 15. Targeting nucleic acids in bacteria with synthetic ligands

    Author : Abbas Nikravesh; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : There is a need for new antibacterial agents, and one attractive strategy is to develop nucleic acid ligands that inhibit pathogen genes selectively. Also, such ligands can be used as molecular biology probes to study gene function and nucleic acid structures. READ MORE