Search for dissertations about: "nucleotide pool"

Showing result 1 - 5 of 24 swedish dissertations containing the words nucleotide pool.

2. 1. Effects of Antioxidants and Pro-oxidants on Oxidative Stress and DNA Damage using the Comet Assay : Studies on Blood Cells from Type 2 Diabetes Subjects and Mouse Lymphoma Cells

   Author : Rikard Åsgård; Björn Hellman; Leif Busk; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; metabolic syndrome; fruit and vegetable intake; plasma antioxidants; beta-carotene; alpha-tocopherol; inflammation; oxidative DNA damage; lipid peroxidation; mouse lymphoma assay; ROS; nucleotide pool; viability; DNA dye; Toxicology; Toxikologi;

   Abstract : Diet and oral supplements comprise two distinct sources of antioxidants known to prevent oxidative stress. Beneficial effects from antioxidants have been seen for patients at risk for type 2 diabetes.The aim of this thesis was to evaluate the positive effects of antioxidants against oxidative stress and DNA damage in type 2 diabetes subjects. READ MORE

4. 2. Exploiting nucleotide metabolism to improve cancer therapy : by targeting dUTPase, dCTPase and NUDT15

   Author : Anna Hagenkort; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Synthetic nucleobase- and nucleoside-analogs have stood the test of time and remain a cornerstone in the treatment regimen against various forms of cancer. Due to their resemblance to endogenous nucleotides these antimetabolites interfere with cellular pathways, including nucleotide metabolism, as well as DNA and RNA synthesis. READ MORE

5. 3. Roles of NUDT5 and NUDT15 beyond oxidized nucleotide sanitation and their potential as therapeutic targets

   Author : Nicholas Carl Kristoffer Valerie; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : The nucleotide precursor pool is readily susceptible to numerous sources of modification and damage, including alkylation, deamination and oxidation/nitrosylation, among others; most of which have deleterious effects on nucleic acid integrity and cellular fitness. In addition to DNA repair mechanisms, these metabolic byproducts are kept in check by so-called sanitation or “housekeeping” enzymes, chief among them the NUDIX hydrolase superfamily. READ MORE

6. 4. Parallel target selection by trinucleotide threading

   Author : Pawel Zajac; Joakim Lundeberg; Afshin Ahmadian; Mats Nilsson; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; trinucleotide threading; multiplex amplification; expression profiling; microarray; generic tag; short tandem repeat; microsatellite; electrophoresis; single nucleotide polymorphism; allelotyping; 454; Pyrosequencing; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Abstract : DNA is the code for all life. Via intermediary RNA the information encoded by the genome is relayed to proteins executing the various functions in a cell. Together, this repertoire of inherently linked biological macromolecules determines all characteristics and features of a cell. READ MORE

7. 5. Interrogation of Nucleic Acids by Parallel Threading

   Author : Erik Pettersson; Joakim Lundeberg; Afshin Ahmadian; Ann-Christine Syvänen; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genotyping; multiplex amplification; trinucleotide threading; single nucleotide polymorphism; genotype calling; Expectation-Maximization; protein-epitope signature tag; expression profiling; Human Protein Atlas; pooled genomic DNA; Pyrosequencing; 454; allelotyping; association studies; bioinformatics; Bioengineering; Bioteknik;

   Abstract : Advancements in the field of biotechnology are expanding the scientific horizon and a promising era is envisioned with personalized medicine for improved health. The amount of genetic data is growing at an ever-escalating pace due to the availability of novel technologies that allow massively parallel sequencing and whole-genome genotyping, that are supported by the advancements in computer science and information technologies. READ MORE