Search for dissertations about: "over-expression"

Showing result 1 - 5 of 138 swedish dissertations containing the word over-expression.

  2. 1. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

    Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE

  4. 2. Extracellular serine proteases as virulence factors in nematophagous fungi: Molecular characterization and functional analysis of the PII protease in Arthrobotrys oligospora

    Author : Johan Åhman; Biologiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; Aspergillus niger.; Heterologous expression; Over-expression; Gene-disruption; Transformation system; Serine protease; Biological control; Arthrobotrys oligospora; Fungi; Nematophagous; Biologi;

    Abstract : Nematophagous fungi are parasites of nematodes, thus they have been considered and tested for biological control of parasitic nematodes, but so far with limited success. Due to the fact that the nematode cuticle is composed of proteins it is likely that fungal extracellular proteases are involved in the infection of nematodes. READ MORE

  5. 3. Engineering membrane proteins for production and topology

    Author : Stephen Toddo; Daniel Daley; Roslyn Bill; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; Biochemistry; biokemi;

    Abstract : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. READ MORE

  6. 4. Role of NFAT (nuclear factor of activated T cells) in vascular smooth muscle

    Author : Lisa Berglund; Malmö Institutionen för kliniska vetenskaper; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; vascular smooth muscle; proliferation; A-285222; osteopontin; AIF-1; diabetes; vascular disease; calcium; glucose; NFAT;

    Abstract : The calcium-dependent transcription factor NFAT was first described as a key player in immune cell regulation. Since then, NFAT signaling has been shown to be crucial for normal vascular development during embryogenesis. READ MORE

  7. 5. Metabolic Engineering of the Pentose Phosphate Pathway of Xylose Fermenting Saccharomyces cerevisiae

    Author : Björn Johansson; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Chemical technology and engineering; xylulokinase; pentose phosphate pathway; genetic engineering; Saccharomyces cerevisiae; xylose; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : The aim of the work presented in this thesis is the improvement of xylose fermentation performance of recombinant Saccharomyces cerevisiae strains expressing XYL1 and XYL2 from Pichia stipitis, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH). A recombinant strain of S. READ MORE