Search for dissertations about: "oxylipin"

Showing result 1 - 5 of 9 swedish dissertations containing the word oxylipin.

  1. 1. Expression of Manganese Lipoxygenase and Site-Directed Mutagenesis of Catalytically Important Amino Acids : Studies on Fatty Acid Dioxygenases

    Author : Mirela Cristea; Ernst H. Oliw; Chao Su; Olof Rådmark; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; Dioxygenase; Gaeumannomyces graminis; lipoxygenase; Magnaporthe grisea; oxylipin; Pichia pastoris; hydroperoxide isomerase; polyunsaturated fatty acids; Farmaceutisk farmakologi; Pharmaceutical pharmacology; Farmaceutisk farmakologi;

    Abstract : Polyunsaturated fatty acids can be bioactivated by two families of dioxygenases, which either contain non-heme iron (lipoxygenases) or heme (cyclooxygenases, linoleate diol synthases and α-dioxygenases).Lipoxygenases and their products play important roles in the pathophysiology of plants and fungi. READ MORE

  2. 2. Catalytic and Structural Properties of Heme-containing Fatty Acid Dioxygenases : Similarities of Fungal Dioxygenases and Cyclooxygenases

    Author : Ulrike Garscha; Ernst Oliw; Per-Johan Jakobsson; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; dioxygenase; prostaglandin H synthase; Gaeumannomyces graminis; Aspergilli; hydroperoxide isomerase; linoleate diol synthase; cytochrome P450; oxylipin; Pharmaceutical pharmacology; Farmaceutisk farmakologi; Farmaceutisk farmakologi; Pharmaceutical Pharmacology;

    Abstract : 7,8-Linoleate diol synthase (7,8-LDS) of the take-all pathogen of wheat, Gaeumannomyces graminis, converts linoleic acid to 8R-hydroperoxyoctadecadienoic acid (8-HPODE) by 8-dioxygenase activity (8-DOX), and further isomerizes the hydroperoxide to 7S,8S-dihydroxyoctadecadienoic acid (7,8-DiHODE) by hydroperoxide isomerase activity. Sequence alignment showed homology to prostaglandin H synthase (PGHS), and both enzymes share structural and catalytic properties. READ MORE

  3. 3. Novel Fatty Acid Dioxygenases of Human and Plant Pathogenic Fungi : Studies by Gene Deletion and Expression

    Author : Fredrik Jernerén; Ernst Oliw; Ivo Feussner; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; aspergilli; dioxygenase; oxygenase; Magnaporthe oryzae; Gaeumannomyces graminis; Lasiodiplodia theobromae; jasmonic acid; linoleate diol synthase; cyclooxygenase; prostaglandin H synthase; cytochrome P450; oxylipin; hydroperoxide isomerase; allene oxide synthase; Pharmaceutical pharmacology; Farmaceutisk farmakologi; Biochemical Pharmacology; Biokemisk farmakologi;

    Abstract : The dioxygenase-cytochrome P450 fusion proteins (DOX-CYP) comprise a heme-containing enzyme family that shares structural and catalytic properties with mammalian prostaglandin H (PGH) synthases. 7,8-Linoleate diol synthase (7,8-LDS) of Gaeumannomyces graminis was first characterized, and DOX-CYP enzymes are of mechanistic and biological interest. READ MORE

  4. 4. Characterization of two novel fatty acid dioxygenases : Linoleate diol synthase and manganese lipoxygenase

    Author : Chao Su; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical biosciences; Dioxygenase; hemeprotein; lipoxygenase; metalloenzyme; oxylipin; fatty acid; manganese-protein; Gaeumannomyces graminis; Farmaceutisk biovetenskap; Biopharmacy; Biofarmaci; galenisk farmaci; Pharmaceutics;

    Abstract : Two classes of fatty acid dioxygenases, PGH synthases (hemeproteins) and lipoxygenases (non-heme iron proteins), are of eminent pharmacological importance. This thesis describes a PGH synthase-related dioxygenase designated linoleate diol synthase (LDS) and the first native occurring manganese-containing lipoxygenase. READ MORE

  5. 5. The structural basis for the catalytic specificity of manganese lipoxygenases : 3D structure analysis of the lipoxygenase of Magnaporthe oryzae

    Author : Anneli Wennman; Ernst H. Oliw; Marcia Newcomer; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; oxylipin; lipoxygenase; crystal structure; crystallography; HPLC; mass spectrometry; yeast; site-directed mutagenesis; fungi; Biokemisk farmakologi; Biochemical Pharmacology;

    Abstract : Lipoxygenases (LOX) catalyze regio- and stereospecific oxygenation of polyunsaturated fatty acids to hydroperoxides. These hydroperoxides are further metabolized to leukotrienes and lipoxins in mammals, and are involved in asthma and inflammation. LOX of animals and plants contain iron as catalytic metal (FeLOX). READ MORE