Search for dissertations about: "padlock probe"

Showing result 1 - 5 of 23 swedish dissertations containing the words padlock probe.

  1. 1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mats Nilsson; Uppsala universitet.; [1998]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. READ MORE

  2. 2. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Sara Henriksson; Uppsala universitet.; [2010]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; MEDICINE Morphology; cell biology; pathology Pathology Molecular medicine; MEDICIN Morfologi; cellbiologi; patologi Patologi Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. READ MORE

  3. 3. Genotyping RNA and DNA using padlock probes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Dan-Oscar Antson; Uppsala universitet.; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. READ MORE

  4. 4. Padlock Probe-Based Assays for Molecular Diagnostics

    University dissertation from Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Author : Anja Mezger; Stockholms universitet.; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Padlock probes; rolling circle amplification; molecular diagnostics; in situ mutation detection; biokemi; Biochemistry;

    Abstract : Treatment success often depends on the availability of accurate and reliable diagnostic assays to guide clinical practitioners in their treatment choices. An optimal test must excel in specificity and sensitivity, and depending on the application area time, low-cost and simplicity are equally important. READ MORE

  5. 5. Readout Strategies for Biomolecular Analyses

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Jenny Göransson; Uppsala universitet.; [2008]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; Selector probe; Rolling circle amplification; Single molecule detection; Amplified single molecule; Random array; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ;

    Abstract : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. READ MORE