Search for dissertations about: "padlock probe"

Showing result 1 - 5 of 26 swedish dissertations containing the words padlock probe.

  1. 1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    Author : Mats Nilsson; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. READ MORE

  2. 2. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

    Author : Sara Henriksson; Mats Nilsson; Ulf Landegren; Harvest Gu; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. READ MORE

  3. 3. Padlock Probe-Based Nucleic Acid Amplification Tests : Point-of-care Diagnostics of Infectious Diseases

    Author : Sibel Ciftci; Mats Nilsson; Jonas Klingström; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Nucleic Acid Amplification; Isothermal Amplification Methods; Padlock Probes; Rolling Circle Amplification; Molecular Diagnostics; Infectious Disease Diagnostics; Virus; Point-of-Care; biokemi; Biochemistry;

    Abstract : Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. READ MORE

  4. 4. Genotyping RNA and DNA using padlock probes

    Author : Dan-Oscar Antson; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. READ MORE

  5. 5. Padlock Probe-Based Assays for Molecular Diagnostics

    Author : Anja Mezger; Mats Nilsson; Paul Blainey; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Padlock probes; rolling circle amplification; molecular diagnostics; in situ mutation detection; biokemi; Biochemistry;

    Abstract : Treatment success often depends on the availability of accurate and reliable diagnostic assays to guide clinical practitioners in their treatment choices. An optimal test must excel in specificity and sensitivity, and depending on the application area time, low-cost and simplicity are equally important. READ MORE