Search for dissertations about: "padlock probe"

Showing result 11 - 15 of 27 swedish dissertations containing the words padlock probe.

2. 11. Detection of Biomolecules Using Volume-Amplified Magnetic Nanobeads

   Author : Teresa Zardán Gómez de la Torre; Maria Strømme; Peter Svedlindh; Mikkel Fougt Hansen; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Magnetic biosensor; magnetic nanobeads; Brownian relaxation; padlock probe; rolling circle amplification; DNA detection; protein detection; Engineering Science with specialization in Nanotechnology and Functional Materials; Teknisk fysik med inriktning mot nanoteknologi och funktionella material;

   Abstract : This thesis describes a new approach to biomolecular analysis, called the volume-amplified magnetic nanobead detection assay (VAM-DNA). It is a sensitive, specific magnetic bioassay that offers a potential platform for the development of low-cost, easy-to-use diagnostic devices. READ MORE

4. 12. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

5. 13. Molecular Tools for Biomarker Detection

   Author : Lei Chen; Ulf Landegren; Niko Hildebrandt; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; padlock probe;

   Abstract : The advance of biological research promotes the emerging of new methods and solutions to answer the biological questions. This thesis describes several new molecular tools and their applications for the detection of genomic and proteomic information with extremely high sensitivity and specificity or simplify such detection procedures without compromising the performance. READ MORE

6. 14. Ligation-mediated Molecular Analysis of Influenza Subtypes, Splicing and Protein Glycosylation

   Author : Tim Conze; Ulf Landegren; Mats Nilsson; Ola Söderberg; Jörn Glökler; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ligase; proximity ligation; gastric cancer; glycosylation; alternative splicing; avian influenza; padlock probe; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Abstract : Binder-based assays are employed throughout the life sciences. Powerful signal amplification techniques have enabled detection of very rare molecule species diluted in simple buffers. Unspecific binding of primary binders leads to increased background in more complex samples. READ MORE

7. 15. Nucleic Acid Based Pathogen Diagnostics

   Author : Michael S. Akhras; Pål Nyrén; Nader Pourmand; Mats Nilsson; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; pathogen diagnostics; antibiotic resistance; connector inversion probes CIPer ; human papillomavirus HPV ; genotyping; global health diagnostic forum; molecular inversion probe MIP ; neisseria gonorrhoeae; padlock probes; pyrosequencing; Biochemistry; Biokemi;

   Abstract : Pathogenic organisms are transmitted to the host organism through all possible connected pathways, and cause a myriad of diseases states. Commonly occurring curable infectious diseases still impose the greatest health impacts on a worldwide perspective. READ MORE