Search for dissertations about: "padlock probe"
Showing result 6 - 10 of 27 swedish dissertations containing the words padlock probe.
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6. Readout Strategies for Biomolecular Analyses
Abstract : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. READ MORE
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7. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection
Abstract : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. READ MORE
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8. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes
Abstract : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. READ MORE
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9. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification
Abstract : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. READ MORE
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10. Visualizing Interacting Biomolecules In Situ
Abstract : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. READ MORE