Search for dissertations about: "phosphotransferase system"

Showing result 1 - 5 of 8 swedish dissertations containing the words phosphotransferase system.

  2. 1. Growth rate control of periplasmic product retention in Escherichia coli

    Author : Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Abstract : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. READ MORE

  4. 2. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Author : Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Abstract : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. READ MORE

  5. 3. Semliki forest virus-derived packaging system for production of retroviral vectors

    Author : Ke-Jun Li; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Moloney murine leukemia virus; Semliki Forest virus; gene therapy; retroviral vector; intron; human bone marrow stromal cell;

    Abstract : Gene therapy is a treatment of disease by transfer of genetic material into somatic cells of an individual. Successful gene therapy depends on a delivery system which can efficiently transfer the gene into target cells and lead to high level expression of the gene. READ MORE

  6. 4. Stress response and virulence in Vibrio anguillarum

    Author : Barbara Weber; Debra L. Milton; James D. Oliver; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Vibrio anguillarum; quorum sensing; Type VI secretion; stress response; virulence; skin colonization; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : Bacteria use quorum sensing, a cell to cell signaling mechanism mediated by small molecules that are produced by specific signal molecule synthases, to regulate gene expression in response to population density. In Vibrio anguillarum, the quorum-sensing phosphorelay channels information from three hybrid sensor kinases VanN, VanQ, CqsS that sense signal molecules produced by the synthases VanM, VanS and CqsA, onto the phosphotransferase VanU, to regulate activity of the response regulator VanO. READ MORE

  7. 5. Streptococcus pyogenes secreted enzymes interacting with the human host

    Author : Mattias Collin; Infektionsmedicin; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; RNA fingerprinting; Streptococcus pyogenes; Clinical biology; immunoglobulins; Klinisk biologi; autoimmunity; endoglycosidase; cysteine proteinase; glycosylation;

    Abstract : Streptococcus pyogenes is one of the most common bacteria infecting humans. One of the factors contributing to the disease-causing properties is the secreted streptococcal cysteine proteinase, SpeB, which degrades several host proteins in connective tissue and circulation. READ MORE