Search for dissertations about: "plasmid stability"

Showing result 1 - 5 of 16 swedish dissertations containing the words plasmid stability.

  2. 1. Global regulatory factors that impact metabolic and lifestyle choices in Pseudomonas putida

    Author : Lisa Wirebrand; Victoria Shingler; Matthew Francis; Carlos Balsalobre; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Pseudomonas putida; c-di-GMP; motility; ppGpp; DksA; biofilm dispersal; transcriptional and translational regulation; dmp-system; phenol catabolism; carbon repression control; plasmid stability;

    Abstract : Pseudomonas putida strains have a broad metabolic capacity and are innately resistant to many harmful substances – properties that make them of interest for a number of industrial and biotechnological application. They can rapidly adapt to changes in physico-chemical parameters in the soil and water environments they naturally inhabit. READ MORE

  4. 2. Structure, function and metabolic stability of antisense RNAs

    Author : Fredrik Söderbom; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microbiology; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Abstract : Antisense RNAs usually are small, highly structured, unstable RNAs that control a varietyof different biological processes. They exert their effect by binding to complementarytarget RNAs and thereby inhibit their functions. READ MORE

  5. 3. Chitosan Polyplexes as Non-Viral Gene Delivery Systems : Structure-Property Relationships and In Vivo Efficiency

    Author : Magnus Köping-Höggård; Per Artursson; Jean-Paul Behr; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutics; gene therapy; gene delivery; non-viral; polyplex; chitosan; chitosan oligomers; polyethylenimine; plasmid DNA; lung; Galenisk farmaci; Pharmaceutics; Galenisk farmaci;

    Abstract : The subject of this thesis was to develop and optimize delivery systems for plasmid DNA (pDNA) based on biocompatible polymers, in particular chitosan, suitable for non-viral gene therapy. At the onset of this thesis, studies had reported conflicting results on the efficiency of chitosan-based gene delivery systems. READ MORE

  6. 4. Prediction, Design and Determination of Protein Structures

    Author : Sebastian Rämisch; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein design; Rosetta; Fold-and-Dock; leucine rich repeat proteins; coiled-coils; molecular replacement; ab initio phasing; temperature stability; fluorescent proteins;

    Abstract : The three-dimensional structure of protein is encoded in its amino acid sequence. Modern structure prediction algorithms make it possible to predict the structure of small proteins using sequence information alone. READ MORE

  7. 5. Mechanisms and Dynamics of Carbapenem Resistance in Escherichia coli

    Author : Marlen Adler; Linus Sandegren; Josep Casadesús; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; carbapenem; antibiotic resistance; fitness cost; ESBLs; penicillin-binding proteins; gene amplification;

    Abstract : The emergence of extended spectrum β-lactamase (ESBL) producing Enterobacteriaceae worldwide has led to an increased use of carbapenems and may drive the development of carbapenem resistance. Existing mechanisms are mainly due to acquired carbapenemases or the combination of ESBL-production and reduced outer membrane permeability. READ MORE