Search for dissertations about: "populus tremula x populus tremuloides"

Showing result 1 - 5 of 26 swedish dissertations containing the words populus tremula x populus tremuloides.

2. 1. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

   Author : Åsa Kallas; KTH; []
   Keywords : xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

   Abstract : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. READ MORE

4. 2. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

   Author : Maria Henriksson; Tuula Teeri; KTH; []
   Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

   Abstract : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. READ MORE

5. 3. Cellulose synthases in Populus- identification, expression analyses and in vitro synthesis

   Author : Soraya Djerbi; Tuula Teeri; Petter Gustafsson; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; cellulose synthase; Populus; secondary cell wall; gene upregulation; cell suspension culture; stationary phase; Bioengineering; Bioteknik;

   Abstract : Cellulose is a biopolymer of great relevance in the plant cell walls, where it constitutes the most important skeletal component. Cellulose is also an important raw material in the pulp- and paper, forest, and textile industries, among others. READ MORE

6. 4. Composition of lignin in outer cell-wall layers

   Author : Maria Christiernin; Gunnar Henriksson; Gösta Brunow; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Lignin; thioacidolysis; primary wall; middle lamella; Populus balsamifera; Populus tremula L. x P. tremuloides Michx.; Picea abies; Picea glauca; Cellulose and paper engineering; Cellulosa- och pappersteknik;

   Abstract : The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. READ MORE

7. 5. Structural Studies of a Xyloglucan Endotransglycosylase from Populus tremula x tremuloides and Three Conserved Hypothetical Proteins from Mycobacterium tuberculosis

   Author : Patrik Johansson; Alwyn Jones; Sherry Mowbray; Pedro Alzari; Uppsala universitet; []
   Keywords : Cell and molecular biology; xyloglucan endotransglycosylase; Mycobacterium tuberculosis; epoxide hydrolase; fatty acid metabolism; fatty acid oxidation; crystallography; Cell- och molekylärbiologi;

   Abstract : This thesis describes the structural studies of four different proteins from two organisms. Xyloglucan endotransglycosylases, XETs, are involved in plant cell wall expansion and remodeling by splitting and reconnecting xyloglucan-cellulose crosslinks. The first crystal structure of a XET enzyme has been determined to 1.8 Å. READ MORE