Search for dissertations about: "primary human hepatocytes"

Showing result 1 - 5 of 39 swedish dissertations containing the words primary human hepatocytes.

2. 1. In vitro and in silico Predictions of Hepatic Transporter-Mediated Drug Clearance and Drug-Drug Interactions in vivo

   Author : Anna Vildhede; Per Artursson; Maria Karlgren; Maria Kjellsson; Ulf Norinder; Kim Brouwer; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; OATP1B1; OATP1B3; OATP2B1; NTCP; drug transporters; human hepatocytes; atorvastatin; pitavastatin; proteomics; sandwich-cultured human hepatocytes; SCHH; mechanistic modeling; in vitro-in vivo extrapolation; transport inhibition; hepatic uptake; hepatocyte isolation; transporter contribution; Biopharmaceutics; Biofarmaci;

   Abstract : The liver is the major detoxifying organ, clearing the blood from drugs and other xenobiotics. The extent of hepatic clearance (CL) determines drug exposure and hence, the efficacy and toxicity associated with exposure. Drug-drug interactions (DDIs) that alter the hepatic CL may cause more or less severe outcomes, such as adverse drug reactions. READ MORE

4. 2. Use of primary human hepatocytes for the elucidation of bile acid synthesis

   Author : Ewa C S Ellis; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : In the present investigation we cultured primary human hepatocytes on matrigel under serum-free conditions and studied bile acid formation. The following observations were made: · The major steroid products excreted from primary human hepatocytes into the culture medium were cholic acid, CA (70%) and chenodeoxycholic acid, CDCA (25%) conjugated with glycine or taurine. READ MORE

5. 3. Proteomic and Functional Analysis of In Vitro Systems for Studies of Drug Disposition in the Human Small Intestine and Liver

   Author : Magnus Ölander; Per Artursson; Pär Matsson; Edward L. LeCluyse; Volker Lauschke; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; drug disposition; ADMET; drug transport; drug metabolism; hepatotoxicity; small intestine; liver; caco-2; human hepatocytes; cryopreservation; apoptosis; liver zonation; non-parenchymal cells; Farmaceutisk vetenskap; Pharmaceutical Science;

   Abstract : To reach the bloodstream, an orally administered drug must be absorbed through the small intestine and avoid extensive clearance in the liver. Estimating these parameters in vitro is therefore important in drug discovery and development. READ MORE

6. 4. Fatty acids induce potentially atherogenic changes in extracellular matrix proteoglycans

   Author : Mariam Rodríguez-Lee; Göteborgs universitet; []
   Keywords : proteoglycans; glycosaminoglycans; fatty acids; smooth muscle cells; hepatocytes; LDL binding; remnant clearance; insulin resistance; type 2 diabetes;

   Abstract : Insulin resistance and type 2 diabetes (T2D) are associated with an increased prevalence of atherosclerotic cardiovascular disease (CVD). A key step in the initiation of atherogenesis is retention of low density lipoproteins (LDL) in the intima by chondroitin sulfate (CS)-rich proteoglycans (PG). READ MORE

7. 5. Charcterization of biotransformation systems in human cells

   Author : Therese Söderdahl; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Efforts are continuously being made to improve test procedures so that predictions of toxicity can be made earlier and more accurately. Understanding mechanisms of toxicity is an important part of improving safety assessment, but for this, simplified methods and models to screen for mechanisms associated with toxicity are required. READ MORE