Search for dissertations about: "production of recombinant lipase in pichia pastoris"

Found 1 swedish dissertation containing the words production of recombinant lipase in pichia pastoris.

2. 1. Expression and Mutagenesis studies of Candida antactica lipase B

   Author : Johanna C. Rotticci-Mulder; KTH; []
   Keywords : Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

   Abstract : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. READ MORE