Search for dissertations about: "protein characterization"

Showing result 11 - 15 of 788 swedish dissertations containing the words protein characterization.

  2. 11. Protein production, characterization and structure determination in structural genomics

    Author : Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

    Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE

  4. 12. Infrared spectroscopy : a tool for protein characterization

    Author : Chenge Li; Andreas Barth; Silvia Maria Doglia; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; biokemi;

    Abstract : Infrared (IR) spectroscopy, which belongs to vibrational spectroscopy, detects the vibrations of molecules, for example, proteins. The absorption of the peptide group gives rise to 9 characteristic bands in the infrared region, named A, B, I-VII, with a decreasing energy or wavenumber (cm-1). READ MORE

  5. 13. Mapping and annotating the mammalian body-wide protein-coding gene expression

    Author : Max Karlsson; Mathias Uhlén; Linn Fagerberg; Julio Saez-Rodriguez; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein; protein-coding; genes; annotation; atlas; scRNA-Seq; RNA-Seq; transcriptomics; proteomics; Biotechnology; Bioteknologi;

    Abstract : A central aim of fundamental research is to create conditions necessary for fueling further research and innovation. Our understanding of basic biology is central for future developments of tools for diagnosing, monitoring, and treating disease. READ MORE

  6. 14. Functional Characterization of the Cellular Protein p32 : A Protein Regulating Adenovirus Transcription and Splicing Through Targeting of Phosphorylation

    Author : Christina Öhrmalm; Göran Akusjärvi; Neus Visa; Uppsala universitet; []
    Keywords : Cell and molecular biology; Adenovirus; p32; ASF SF2; E4-ORF4; Splicing; Transcription; CTD; RNA Pol II; Cell- och molekylärbiologi;

    Abstract : Cellular processes involved in the conversion of the genetic information from DNA into a protein are often regulated by reversible phosphorylation reactions. By modulating the phosphorylated status of key proteins their activity can either be enhanced or repressed. READ MORE

  7. 15. Genome-wide structural and functional protein characterization by ab initio protein structure prediction

    Author : Lars Malmström; Avdelningen för Biomedicinsk teknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biomedicinska vetenskaper; Biomedical sciences; Saccharymyces cerevisiae; Ab initio protein structure prediction; Protein annotation;

    Abstract : Very little is known about a considerable part of all proteins and it is time consuming and expensive to study each individual protein to determine its function, structure and cellular role. Proteins retain structural, functional and sequential characteristics from ancestral proteins and hence two proteins that share a common ancestor, i.e. READ MORE