Search for dissertations about: "protein immobilization."

Showing result 1 - 5 of 71 swedish dissertations containing the words protein immobilization..

  2. 1. Immobilization and Interaction of Biomolecules at the Solid-Liquid Interface : Applications to Solid-Phase Analytical Techniques

    Author : Ulf Jönsson; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein immobilization; silica; Immunoglobulins; Protein A; Fibronectin; chemical-vapour deposition; silane; ellipsometry; radiolabeling; contact-angle measurement; solid-phase analytical techniques;

    Abstract : The development of solid-phase-analytical techniques based on surface-concentration-measuring devices requires a stable and reproducible immobilization of biomolecules on well-characterized solid surfaces. This study reports on the immobilization of Immunoglobulin G, Protein A and Fibronectin on chemically modified silica surfaces. READ MORE

  4. 2. Biosensor surface chemistry for oriented protein immobilization and biochip patterning

    Author : Emma Ericsson; Karin Enander; Sofia Svedhem; Linköpings universitet; []
    Keywords : Biochip; biosensor; hydrogel; ligand; orientation; patterning; photochemistry; protein immobilization; surface chemistry; self-assembled monolayer; SAM;

    Abstract : This licentiate thesis is focused on two methods for protein immobilization to biosensor surfaces for future applications in protein microarray formats. The common denominator is a surface chemistry based on a gold substrate with a self-assembled monolayer (SAM) of functionalized alkanethiolates. READ MORE

  5. 3. Macromolecules at Interfaces

    Author : Helén Larsericsdotter; Sven Oscarsson; Jos Buijs; Willem Norde; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein; Adsorption; Immobilization; Surface; Surface Plasmon Resonance; SPR; Mass Spectrometry; MS; Differential Scanning Calorimetry; DSC; FT ICR; Lysozyme; Bovine Serum Albumin; BSA; Myoglobin; Globular; Kemi; Chemistry; Kemi;

    Abstract : In this thesis, the structure and stability of globular proteins adsorbed onto nanometer-sized hydrophilic silica particles were investigated using differential scanning calorimetry (DSC), hydrogen/deuterium exchange (HDX), and mass spectrometry (MS). The adsorption process itself was characterized with fluorescence and absorption spectroscopy and surface plasmon resonance (SPR). READ MORE

  6. 4. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Author : Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Abstract : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. READ MORE

  7. 5. Immobilization of feruloyl esterases in mesoporous silica

    Author : Christian Thörn; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Mesoporous materials; Feruloyl esterases; Protein modeling; Biocatalysis; QCM-D; Hydroxycinnamic acids; Enzyme immobilization; Silica;

    Abstract : Feruloyl esterases are a class of enzymes that can be used as biocatalysts for the modification of bioactive hydroxycinnamic acids. In industry, enzyme immobilization is often necessary to stabilize the enzymes and to allow their reuse. READ MORE