Search for dissertations about: "protein localization"

Showing result 11 - 15 of 484 swedish dissertations containing the words protein localization.

2. 11. Mapping the human proteome using bioinformatic methods

   Author : Linn Fagerberg; Mathias Uhlén; Alvis Brazma; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteome; transcriptome; bioinformatics; membrane protein prediction; subcellular localization; protein expression level; cell line; immunohistochemistry; immunofluorescence; Bioinformatics; Bioinformatik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : The fundamental goal of proteomics is to gain an understanding of the expression and function of the proteome on the level of individual proteins, on the level of defined cell types and on the level of the entire organism. In this thesis, the human proteome is explored using membrane protein topology prediction methods to define the human membrane proteome and by global protein expression profiling, which relies on a complex study of the location and expression levels of proteins in tissues and cells. READ MORE

4. 12. Development of DNA-based methods for analysis of protein interactions

   Author : Axel Klaesson; Ola Söderberg; Mats Nilsson; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

   Abstract : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. READ MORE

5. 13. Purine and Pyrazolopyrimidine Derivatives: Design and Synthesis of Chemical Tools for Biological Applications

   Author : David Bliman; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; purine; pyrazolopyrimidine; caged compounds; protein kinases; protein-protein interactions; fluorescence; organic synthesis;

   Abstract : Purines can be found in a multitude of naturally occurring compounds with a range of functions. This thesis describes the design and synthesis of purines and structurally related pyrazolopyrimidine derivatives intended for biological applications. READ MORE

6. 14. Identification of SLiMs: Mapping and characterizing motif-based protein interactions

   Author : Muhammad Ali; Ylva Ivarsson; Shoshana Wodak; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Phage display; protein-protein interactions; short linear motifs; PDZ; FERM; affinity; x-ray crystallization; molecular docking; NLS;

   Abstract : During the last twenty years it has become evident that about 35-40% of amino acids in the proteome are in regions that have evolved to remain unstructured. These intrinsically disordered regions contain short linear motifs (SLiMs), which serve as docking sites for protein-protein interactions. READ MORE

7. 15. Antibody-based subcellular localization of the human proteome

   Author : Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

   Abstract : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. READ MORE