Search for dissertations about: "protein production rate"

Showing result 1 - 5 of 157 swedish dissertations containing the words protein production rate.

2. 1. Proteome wide protein production

   Author : Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
   Keywords : protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

   Abstract : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. READ MORE

4. 2. Enhancing membrane and secretory protein production yields in Escherichia coli

   Author : Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

   Abstract : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. READ MORE

5. 3. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Author : Zihe Liu; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Abstract : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. READ MORE

6. 4. Membrane and secretory protein production in Escherichia coli

   Author : Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Abstract : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. READ MORE

7. 5. Protein production in the E. coli cell envelope

   Author : Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

   Abstract : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. READ MORE