Search for dissertations about: "protein purification"

Showing result 16 - 20 of 265 swedish dissertations containing the words protein purification.

  2. 16. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    Author : Viveka Dolby; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Abstract : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. READ MORE

  4. 17. Purification, characterization and immunological studies of rat urinary proteins causing allergy in humans

    Author : Cecilia Bayard; Gabriel Peltre; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; allergen; laboratory animal allergy; rat urinary protein; Rat n 1.02; a2u-globulin; purification; chemiluminescence; immunologi; Immunology;

    Abstract : Rats are among the most frequently used laboratory animals and allergy to them constitutes a common occupational problem. Approximately 20-30% of the persons engaged in work with laboratory animals acquire symptoms of allergy. READ MORE

  5. 18. Exploration of analytical methods to study motif-mediated host-virus protein-protein interactions

    Author : Eszter Kassa; Ylva Ivarsson; Gilles Travé; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein-protein interaction; short linear motif; affinity purification-mass spectrometry; virus; SARS-CoV-2; TBEV; Analytical Chemistry; Analytisk kemi;

    Abstract : Proteins are responsible for countless processes in living creatures, but most often they do not perform these tasks alone. Rather, they engage in interactions with other proteins, creating whole protein-protein interaction (PPI) networks. READ MORE

  6. 19. Biosensing and Protein Array Design using Lipid Assemblies

    Author : Charlotte Larsson; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein adsorption; protein arrays; lipid bilayer; SPR; scFv; lipid vesicle; DNA arrays; QCM-D;

    Abstract : Biomolecular sensing often includes immobilization of biomolecules, e.g. DNA, antibodies etc., to solid supports, where they act as selective recognition elements. READ MORE

  7. 20. Protein production, characterization and structure determination in structural genomics

    Author : Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

    Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE