Search for dissertations about: "protein quantification"

Showing result 1 - 5 of 210 swedish dissertations containing the words protein quantification.

  2. 1. High-throughput protein analysis using mass spectrometry-based methods

    Author : Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Keywords : Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Abstract : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. READ MORE

  4. 2. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Author : Malin Mejàre; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Abstract : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. READ MORE

  5. 3. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Author : Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Abstract : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. READ MORE

  6. 4. On the Development of Mucin-based Biomaterial Coatings

    Author : Tomas Sandberg; Karin Caldwell; Jan Carlsson; Thomas Arnebrant; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mucin; Biomaterial; Surface-exposed protein; XPS; Neutrophil; Cell morphology; SEM; QCM-D; Viscoelasticity; Protein-stabilization; HNL; Coating; MG1; BSM; PGM; SEC-MALS-RI; Mucin quantification; Protein adsorption; Ellipsometry; Biomaterials; Biomaterial;

    Abstract : Owing to their key role in mucosal functioning as surface barriers with biospecific interaction potentials, the mucins are interesting candidates for use as surface modifiers in biomaterials applications. In this work, “mild” fractionation procedures were used to prepare mucins of bovine (BSM), porcine (PGM), and human (MG1) origin. READ MORE

  7. 5. Towards specific quantification of exosomes using surface-based sensing

    Author : Deborah Rupert; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; label-free; surface plasmon resonance.; exosome; binding rate; antibody; concentration; nanoparticle tracking analysis; quantification; liposome; cluster of differentiation 63 CD63 ; diffusion; specific detection; mass transport limited conditions; extracellular vesicles; surface-based biosensor;

    Abstract : When exosomes were first observed in 1967, they were believed to be cell debris until extensive studies demonstrated key biological functionalities related to their composition and formation mechanism. Exosomes are nanometer-sized lipid vesicles of endocytic origin, with a membrane consisting of lipids and proteins and encapsulating a cargo composed of soluble proteins and genetic material. READ MORE