Search for dissertations about: "protein release"

Showing result 21 - 25 of 743 swedish dissertations containing the words protein release.

  2. 21. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

  4. 22. Studies of Eosinophil Cationic Protein (ECP) in vivo and in vitro : Impact of Genetic and Posttranslational Modifications

    Author : Jenny Eriksson; Per Venge; Agneta Trulson; Ester Boix; Uppsala universitet; []
    Keywords : Biomedicine; Eosinophil granulocyte; granular protein; ECP; gene polymorphism; cytotoxicity; SELDI-TOF MS; glycosylation; molecular and functional heterogeneity; fibrosis; Biomedicin;

    Abstract : Eosinophil granulocytes are tissue dwelling leukocytes that are implicated in host defence, particularly against helminthic parasites; they also participate in most inflammatory disorders. Although eosinophils have important roles in host defence mechanisms, their actions can also be harmful to the host as in the allergic inflammation where lung epithelium is destructed due to the release of toxic granule proteins. READ MORE

  5. 23. The Protein Traffic on the Ribosome : The Mechanism and Regulation of Protein Synthesis in Prokaryotes

    Author : Andrey Zavialov; Måns Ehrenber; Venki Ramakrishnan; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; yellow; ribosome; translation; Gagarin; nobel prize; Biokemi; Biochemistry; Biokemi;

    Abstract : The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. READ MORE

  6. 24. Antibiotic-induced Bacterial Toxin Release – Inhibition by Protein Synthesis Inhibitors

    Author : Gunilla Hjerdt-Goscinski; Jan Sjölin; Monica Lundholm; Inga Odenholt; Ingrid Nilsson-Ehle; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Communicable diseases; Endotoxin; LPS; exotoxin; SpeA; penicillin-binding protein; aminoglycosides; clindamycin; β-lactam antibiotics; severe sepsis; septic shock; Infektionssjukdomar; Infectious diseases; Infektionssjukdomar;

    Abstract : Toxic products, such as endotoxin from the gram-negative and exotoxin from the gram-positive bacteria, are the most important initiators of the inflammatory host response in sepsis. In addition to antibacterial treatment, numerous attempts have been made to interfere with the exaggerated proinflammatory cascade initiated by the toxins. READ MORE

  7. 25. Engineering of Human Fetal Hemoglobin

    Author : Karin Kettisen; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fetal hemoglobin; protein engineering; recombinant protein production; protein purification; chromatography; oxygen carrier; redox reactions; net surface charge; DNA cleavage;

    Abstract : In blood, the oxygen-transporting protein hemoglobin (Hb) governs the oxygenation of cells and tissues. Naturally, this protein has claimed a place in the center of the research field of artificial oxygen therapeutics. READ MORE