Search for dissertations about: "protein scaffold"

Showing result 1 - 5 of 139 swedish dissertations containing the words protein scaffold.

  2. 1. Molecular principles of protein stability and protein-protein interactions

    Author : Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; Structural biochemistry; Strukturbiokemi;

    Abstract : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. READ MORE

  4. 2. Protein folding without loops and charges

    Author : Martin Kurnik; Mikael Oliveberg; Kevin W. Plaxco; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein folding; folding cooperativity; protein aggregation; protein charges; protein engineering; Biochemistry; biokemi;

    Abstract : Going down the folding funnel, proteins may sample a wide variety of conformations, some being outright detrimental to the organism. Yet, the vast majority of polypeptide molecules avoid such pitfalls. Not only do they reach the native minimum of the energy landscape; they do so via blazingly fast, biased, routes. READ MORE

  5. 3. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Author : Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Abstract : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. READ MORE

  6. 4. Novel binding specificities engineered into the scaffold of a carbohydrate binding module

    Author : Lavinia Cicortas Gunnarsson; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; carbohydrate binding module; binding specificity; selection; combinatorial library; scaffold; protein engineering;

    Abstract : The growing field of biotechnology is in constant need of proteins that can function as recognition tools for separational, analytical and therapeutic purposes. Different molecular engineering approaches are applied on natural proteins in order to create variants with desired properties. READ MORE

  7. 5. Characterization and Engineering of Protein-Protein Interactions Involving PDZ Domains

    Author : Andreas Karlsson; Per Jemth; Daniel Otzen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; intrinsically disordered protein regions; PDZ domain; binding kinetics; protein engineering; interaction mechanism; specificity; PDZbody; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Abstract : The work presented in this thesis has contributed with knowledge to several aspects of protein-protein interaction involving PDZ domains. A substantial amount of our proteome contains regions that are intrinsically disordered but fold upon ligand interaction. READ MORE