Search for dissertations about: "protein stability"

Showing result 1 - 5 of 497 swedish dissertations containing the words protein stability.

  1. 1. Molecular principles of protein stability and protein-protein interactions

    University dissertation from Stockholm : KTH

    Author : Christofer Lendel; Per-Åke Nygren; Christopher M. Dobson; [2005]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; binding thermodynamics; induced fit; molten globule; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; protein structure.; TECHNOLOGY Bioengineering Structural biochemistry; TEKNIKVETENSKAP Bioteknik Strukturbiokemi;

    Abstract : Proteins with highly specific binding properties constitute the basis for many important applications in biotechnology and medicine. Immunoglobulins have so far been the obvious choice but recent advances in protein engineering have provided several novel constructs that indeed challenge antibodies. READ MORE

  2. 2. Perplexing Protein Puzzles

    University dissertation from Department of Biophysical Chemistry, Lund University

    Author : Stina Lindman; [2010]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Abstract : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. READ MORE

  3. 3. Unravelling protein stability and aggregation

    University dissertation from Media-Tryck, Lund University, Sweden

    Author : Tanja Weiffert; [2018-10]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

    Abstract : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. READ MORE

  4. 4. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

    University dissertation from Stockholm : KTH

    Author : Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; [2006]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; TECHNOLOGY Bioengineering Structural biochemistry; TEKNIKVETENSKAP Bioteknik Strukturbiokemi;

    Abstract : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. READ MORE

  5. 5. The birth and growth of the protein folding nucleus Studies of protein folding focused on critical contacts, topology and ionic interactions

    University dissertation from Stockholm : Institutionen för biokemi och biofysik

    Author : Linda Hedberg; Mikael Oliveberg; Christopher Johnson; ; [2008]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein folding; protein stability; two-state folding; chevron plot; transition-state movements; Hammond behaviour; topology; electrostatic interactions; pH dependence; mass-action; protein engineering; pKa; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi; biokemi; Biochemistry;

    Abstract : Proteins are among the most complex molecules in the cell and they play a major role in life itself. The complexity is not restricted to just structure and function, but also embraces the protein folding reaction. READ MORE