Search for dissertations about: "proximity ligation assays"

Showing result 1 - 5 of 26 swedish dissertations containing the words proximity ligation assays.

  2. 1. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    Author : Simon Fredriksson; Larry Gold; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. READ MORE

  4. 2. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

    Author : Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Abstract : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. READ MORE

  5. 3. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

    Author : Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE

  6. 4. Proximity Ligation Assays for Disease Biomarkers Analysis

    Author : Rachel Yuan Nong; Ulf Landegren; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; blood biomarkers; protein interactions; pathway analysis; single molecule; next-generation sequencing; Molekylär medicin; Molecular Medicine;

    Abstract : One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. READ MORE

  7. 5. Visualization of Protein Activity Status in situ Using Proximity Ligation Assays

    Author : Malin Jarvius; Ulf Landegren; Ola Söderberg; Hans van Dam; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ; high content screening; platelet-derived growth factor receptor; rolling circle amplification; protein interactions; in situ PLA; post-translational modifications; drug screening; Molecular biology; Molekylärbiologi; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

    Abstract : In 2001 the human proteome organization (HUPO) was created with the ambition to identify and characterize all proteins encoded in the human genome according to several criteria; their expression levels in different tissues and under different conditions; the sub-cellular localization; post-translational modifications; interactions, and if possible also the relationship between their structure and function.When the knowledge of different proteins and their potential interactions increases, so does the need for methods able to unravel the nature of molecular processes in cells and organized tissues, and ultimately for clinical use in samples obtained from patients. READ MORE