Search for dissertations about: "proximity ligation"

Showing result 1 - 5 of 49 swedish dissertations containing the words proximity ligation.

  1. 1. Proximity Ligation Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Simon Fredriksson; Uppsala universitet.; [2002]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. READ MORE

  2. 2. Proximity Ligation and Barcoding Assays Tools for analysis of proteins and protein complexes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Di Wu; Uppsala universitet.; Uppsala universitet.; [2014]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Abstract : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. READ MORE

  3. 3. Solid-phase Proximity Ligation Assays High-performance and multiplex protein analyses

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Spyros Darmanis; Uppsala universitet.; [2011]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE

  4. 4. Proximity Ligation as a Universal Protein Detection Tool

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mats Gullberg; Uppsala universitet.; [2003]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; aptamer; antibody; molecular beacon; rolling circle amplification; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

    Abstract : Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. READ MORE

  5. 5. Proximity Ligation Assays for Disease Biomarkers Analysis

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Rachel Yuan Nong; Uppsala universitet.; [2011]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; blood biomarkers; protein interactions; pathway analysis; single molecule; next-generation sequencing; Molekylär medicin; Molecular Medicine;

    Abstract : One of the pressing needs in the field of disease biomarker discovery is new technologies that could allow high performance protein analysis in different types of clinical material, such as blood and solid tissues. This thesis includes four approaches that address important limitations of current technologies, thus enabling highly sensitive, specific and parallel protein measurements. READ MORE