Search for dissertations about: "proximity ligation"

Showing result 16 - 20 of 63 swedish dissertations containing the words proximity ligation.

  2. 16. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    Author : Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
    Keywords : Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Abstract : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. READ MORE

  4. 17. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

    Author : Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

    Abstract : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. READ MORE

  5. 18. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Author : Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. READ MORE

  6. 19. Molecular Tools for Detection and Characterization of Proteins and Extracellular Vesicles in Health and Disease

    Author : Ehsan Manouchehri Doulabi; Masood Kamali-Moghaddam; Lucia Mincheva-Nilsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; High-resolution mass spectrometry; Proximity ligation assay; Proximity extension assay; Thymidine kinase 1; in situ PLA; High-throughput.; Molekylär medicin; Molecular Medicine;

    Abstract : Detecting molecules involved in cancer is critical for cancer research and diagnostics. To achieve this goal, sensitive protein detection is essential to improving the chances of finding, verifying, validating and developing valuable biomarkers. READ MORE

  7. 20. Visualizing Interacting Biomolecules In Situ

    Author : Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. READ MORE