Search for dissertations about: "pyrosequencing"

Showing result 6 - 10 of 106 swedish dissertations containing the word pyrosequencing.

  2. 6. Mathematical modelling and analysis of the pyrosequencing reaction system

    Author : Anna Svantesson; Jeanette Hellgren Kotaleski; Anders Lansner; Pål Nyrén; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; bioluminescence; DNA polymerase; DNA sequencing; enzyme kinetics; Klenow fragment; mathematical modelling; Michaelis-Menten; pyrosequencing; Computer science; Datalogi;

    Abstract : .... READ MORE

  4. 7. Method development and applications of Pyrosequencing technology

    Author : Baback Gharizadeh; KTH; []
    Keywords : apyrase; bacterial identification; dATP?S; EST sequencing; fungal identification; human papillomavirus HPV ; microbial and viral typing; multiple sequencing primer method; Pyrosequencing technology; Sequenase; single-stranded DNA-binding protein SS;

    Abstract : The ability to determine nucleic acid sequences is one ofthe most important platforms for the detailed study ofbiological systems. Pyrosequencing technology is a relativelynovel DNA sequencing technique with multifaceted uniquecharacteristics, adjustable to different strategies, formatsand instrumentations. READ MORE

  5. 8. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Author : Jonas Eriksson; KTH; []
    Keywords : bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Abstract : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. READ MORE

  6. 9. Approaches for analysis of mutations and genetic variations

    Author : Afshin Ahmadian; KTH; []
    Keywords : LOH; microsatellite; fragment analysis; DNA sequencing; pyrosequencing; cancer; mutation; variation; SNP; allele-specific extension; apyrase; TECHNOLOGY; TEKNIKVETENSKAP;

    Abstract : Detecting mutations and genomic variations is fundamental indiagnosis, isolating disease genes, association studies,functional genomics and pharmacogenomics. The objective hasbeen to use and further develop a variety of tools andtechnologies to analyze these genetic alterations andvariations. READ MORE

  7. 10. Detection and analysis of genetic alterations in normal skin and skin tumours

    Author : Åsa Sivertsson; KTH; []
    Keywords : single cell; DNA sequencing; p53; mutation; UV; BCC; pyrosequencing; malignant melanoma; N-ras;

    Abstract : The investigation of genetic alterations in cancer-relatedgenes is useful for research, prognostic and therapeuticpurposes. However, the genetic heterogeneity that often occursduring tumour progression can make correct analysischallenging. READ MORE