Search for dissertations about: "rRNA modification"

Showing result 1 - 5 of 16 swedish dissertations containing the words rRNA modification.

  2. 1. Ribosomal RNA Modification Enzymes : Structural and functional studies of two methyltransferases for 23S rRNA modification in Escherichia coli

    Author : Avinash S. Punekar; Maria Selmer; Martino Bolognesi; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; ribosome biogenesis; ribosome assembly; ribosomal RNA; peptidyltransferase center; domain V; post-transcriptional modification; methyltransferases; S-adenosyl-methionine; RlmM; Cm2498; RlmJ; m6A2030; X-ray crystallography; substrate recognition; substrate specificity; catalytic mechanism; evolution; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biokemi; Biochemistry;

    Abstract : Escherichia coli ribosomal RNA (rRNA) is post-transcriptionally modified by site-specific enzymes. The role of most modifications is not known and little is known about how these enzymes recognize their target substrates. READ MORE

  4. 2. High-resolution phenotypic profiling of a eukaryotic ribosome

    Author : Jonathan Esguerra; Göteborgs universitet; []
    Keywords : ribosome; ribosomal protein genes; box C D snoRNA; box H ACA snoRNA; 2’-O-ribose methylation; pseudouridylation; high-resolution phenotypic profiling; chemogenetic profiling; Saccharomyces cerevisiae; rRNA modification;

    Abstract : All living cells contain ribosomes, complex macromolecular assemblies of ribonucleic acid (RNA) and proteins, responsible for synthesizing polypeptides in a process called translation. High-resolution three-dimensional ribosomal structures coupled with biochemical studies have paved the way in understanding the various mechanistic events during protein synthesis. READ MORE

  5. 3. Exotic Ribosomal Enzymology

    Author : Josefine Liljeruhm; Anthony C. Forster; Christophe Danelon; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; rRNA modification; Methyltransferase; RlmM; RlmJ; D-amino acid; Unnatural amino acid; Chromoprotein; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Abstract : This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein complex that catalyzes protein synthesis (translation). The large ribosomal RNAs (23S and 16S rRNAs in E. coli) are post-transcriptionally modified by many specific modification enzymes, yet the functions of the modifications remain enigmatic. READ MORE

  6. 4. Antibiotic Resistance and the Cellular Currency S-adenosyl-methionine : Modification of aminoglycosides and nucleic acids

    Author : Sandesh Kanchugal P; Maria Selmer; Ditlev Brodersen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibiotic resistance; streptomycin; spectinomycin; X-ray crystallography; methyltransferase; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

    Abstract : Streptomycin and spectinomycin are antibiotics that bind to ribosomes and inhibit protein synthesis. Common resistance mechanisms involve enzymatic modification of the two drugs by aminoglycoside nucleotidyltransferases (ANTs). The first part of this thesis covers the structural mechanism of two ANT enzymes. READ MORE

  7. 5. Non-protein-coding RNA : Transcription and regulation of ribosomal RNA

    Author : Stefanie Böhm; Ann-Kristin Östlund Farrants; Brian McStay; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; ribosomal RNA; non-coding RNA; ribosomal genes; rDNA gene; B-WICH; chromatin remodelling; histone modification; Cell Biology; cellbiologi;

    Abstract : Cell growth and proliferation are processes in the cell that must be tightly regulated. Transcription of ribosomal RNA and ribosomal biogenesis are directly linked to cell growth and proliferation, since the ribosomal RNA encodes for the majority of transcription in a cell and ribosomal biogenesis influences directly the number of proteins that are synthesized. READ MORE