Search for dissertations about: "random mutagenesis"

Showing result 1 - 5 of 15 swedish dissertations containing the words random mutagenesis.

  2. 1. Study on the Function of Translation Initiation Factor IF1

    Author : Victor Croitoru; Leif A. Isaksson; Kim Kusk Mortensen; Stockholms universitet; []
    Keywords : translation; initiation factor IF1; mutagenesis; A-site; RNA chaperone; NATURAL SCIENCES; NATURVETENSKAP;

    Abstract : Initiation is the first step in protein biosynthesis representing a fundamental event in cell life which determines fidelity, efficiency and regulation of gene expression. In addition to the ribosome and mRNA, three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. READ MORE

  4. 2. In search of a biosensor for DNT detection : Studies of inducer response and specificity of DntR

    Author : Rosa Lönneborg; Peter Brzezinski; Ray Dixon; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DntR; transcriptional regulation; gfp; nitro-aromatic compounds; LysR family; LTTR; TNT; DNT; FACS; directed evolution; random mutagenesis; recombination; structure determination; biokemi; Biochemistry;

    Abstract : The primary aim of the work presented in this thesis was to change the inducer specificity of the DntR protein in order to improve the response to DNT. The long-term goal is to use this protein in a biosensor for DNT, a signature compound for detection of the explosive TNT. READ MORE

  5. 3. Membrane Protein Tailoring and Structural Studies of Leukotriene C4 Synthase

    Author : Daniel Martinez Molina; Pär Nordlund; Robert M. Stroud; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; membrane proteins; random mutagenesis; detergent screening; Leukotriene C₄ synthase; IMAC; Structural biology; Strukturbiologi; Biochemistry; biokemi;

    Abstract : Despite a dramatic increase in the number of proteins that have been structurally characterized in recent years, there are still less than 200 unique structures of membrane proteins known today. This is only 1% of the total number of unique protein structures found in structural databases worldwide. READ MORE

  6. 4. Metabolic engineering and random mutagenesis for improved xylose utilisation of Saccharomyces cerevisiae

    Author : Fredrik Wahlbom; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk teknik; Biochemical technology; mykologi; virologi; bacteriology; Microbiology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; ethanol; Saccharomyces; xylose; virology; mycology; Mikrobiologi; bakteriologi;

    Abstract : In this thesis I have summarised my work on the analysis and improvement of xylose utilisation by recombinant S. cerevisiae. A metabolic flux model was developed and used to analyse the intracellular fluxes in the recombinant xylose utilising S. cerevisiae TMB 3001 cultivated in chemostat at various dilution rates and xylose/glucose concentrations. READ MORE

  7. 5. Vitreoscilla Haemoglobin. Genetic Fusions and Metabolic Characterisation

    Author : Viktoria Roos; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Metabolism; Biokemi; metabolism; osmotic stress; transcript profiling; DNA microarrays; random elongation mutagenesis; directed evolution; mRNA structure; Vitreoscilla haemoglobin VHb ; Escherichia coli; salt stress.;

    Abstract : Vitreoscilla is an obligate aerobic bacterium living in oxygen-poor environments such as stagnant ponds and decaying vegetable matter. Vitreoscilla expresses a homodimeric haemoglobin (VHb) when subjected to oxygen-limited conditions. READ MORE