Search for dissertations about: "real time pcr qpcr"

Showing result 1 - 5 of 13 swedish dissertations containing the words real time pcr qpcr.

  2. 1. Real-time PCR and its application for protein quantification

    Author : Kristina Lind; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Abstract : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. READ MORE

  4. 2. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Author : Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Keywords : Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Abstract : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. READ MORE

  5. 3. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

    Author : Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
    Keywords : Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

    Abstract : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. READ MORE

  6. 4. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

    Author : Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
    Keywords : Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

    Abstract : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). READ MORE

  7. 5. PCR inhibition mechanisms in forensic DNA analysis

    Author : Maja Sidstedt; Teknisk mikrobiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Abstract : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. READ MORE