Search for dissertations about: "recombinant dna"

Showing result 1 - 5 of 167 swedish dissertations containing the words recombinant dna.

  2. 1. Recombinant Enzymes in Pyrosequencing Technology

    Author : Nader Nourizad; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

  4. 2. Recombinant DNA in Staphylococcus aureus

    Author : Sven Löfdahl; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP;

    Abstract : .... READ MORE

  5. 3. Production and delivery of recombinant subunit vaccines

    Author : Christin Andersson; KTH; []
    Keywords : Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Abstract : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. READ MORE

  6. 4. Recombinant subunit vaccines : protein immunogens

    Author : Sissela Liljeqvist; KTH; []
    Keywords : ;

    Abstract : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. READ MORE

  7. 5. Functional and structural properties of eukaryotic DNA polymerase epsilon

    Author : Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

    Abstract : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. READ MORE