Search for dissertations about: "recombinant expression"

Showing result 1 - 5 of 351 swedish dissertations containing the words recombinant expression.

2. 1. Expression profiling using manifold supports

   Author : Anette Hagberg; Joakim Lundeberg; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Abstract : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. READ MORE

4. 2. Production and delivery of recombinant subunit vaccines

   Author : Christin Andersson; KTH; []
   Keywords : Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

   Abstract : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. READ MORE

5. 3. Recombinant subunit vaccines : protein immunogens

   Author : Sissela Liljeqvist; KTH; []
   Keywords : ;

   Abstract : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. READ MORE

6. 4. Recombinant Enzymes in Pyrosequencing Technology

   Author : Nader Nourizad; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

7. 5. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

   Author : Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

   Abstract : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. READ MORE