Search for dissertations about: "refolding"

Showing result 1 - 5 of 22 swedish dissertations containing the word refolding.

  1. 1. Interaction engineered three-helix bundle domains for protein recovery and detection

    Author : Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

    Abstract : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. READ MORE

  2. 2. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

  3. 3. Rate Limiting Factors For Protein Folding

    Author : Maria Berggård Silow; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; kinetic competition; transient aggregation; diffusion control; reconfiguration time; collapse; osmolytes; broad barriers; Hammond behaviour; two-state; U1A; CI2; Proteins; enzymology; Proteiner; enzymologi;

    Abstract : Abstract. This thesis describes factors that are rate limiting for the folding of two small proteins, U1A and CI2 which fold without accumulating intermediates. The [GdnHCl] dependencies of the unfolding- and refolding kinetics of U1A display downward curvatures. READ MORE

  4. 4. Protein Folding Activity of the Ribosome and Its Implication in Prion Processes

    Author : Yanhong Pang; Suparna Sanyal; Patricia L. Clark; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Ribosome; Protein folding; Prion disease; Antiprion drug; Competitive inhibition; PFAR; Amyloid; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Abstract : How the linear protein chains fold into their three-dimensional active conformation is one of the remaining puzzles of modern science. Other than molecular chaperones, ribosome - the cellular protein synthesis machinery, has also been implicated in protein folding. READ MORE

  5. 5. Biophysical studies of protein folding upon interaction with molecular chaperones

    Author : Satish Babu Moparthi; Uno Carlsson; Per Hammarström; Bengt-Harald Jonsson; Pernilla Wittung‐Stafshede; Linköpings universitet; []

    Abstract : Proteins are biological macromolecules that serve all functions in cells. Every protein consists of a sequence of amino acids that is folded into a three‐dimensional structure to maintain the unique information it contains and to allow the protein to perform its specific actions. READ MORE